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PMID: 16946266 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in Mycobacterium tuberculosis under stress.

Microbiology (Reading, England) ·Vol. 152 ·No. Pt 9 ·2006-09-00 ·Pages 2717-2725

Sirakova TD, Dubey VS, Deb C, Daniel J, Korotkova TA, Abomoelak B, Kolattukudy PE

Abstract

Mycobacterium tuberculosis under stress stores triacylglycerol (TG). There are 15 genes in M. tuberculosis that belong to a novel family of TG synthase genes (tgs), but it is not known which of them is responsible for this accumulation of TG. In this paper, it is reported that M. tuberculosis H37Rv accumulated TG under acidic, static or hypoxic growth conditions, or upon treatment with NO, whereas TG accumulation was drastically reduced in the tgs1 (Rv3130c) disrupted mutant. Complementation with tgs1 restored this TG accumulation. C(26) was a major fatty acid in this TG, indicating that the TGS1 gene product uses C(26) fatty acid, which is known to be produced by the mycobacterial fatty acid synthase. TGS1 expressed in Escherichia coli preferred C(26 : 0)-CoA for TG synthesis. If TG storage is needed for the long-term survival of M. tuberculosis under dormant conditions, the tgs1 product could be a suitable target for antilatency drugs.

MeSH Terms
Diacylglycerol O-Acyltransferase/genetics Escherichia coli/genetics Genes, Bacterial Hydrogen-Ion Concentration Hypoxia Mutation Mycobacterium tuberculosis/drug effects,enzymology,genetics,growth & development,metabolism Nitric Oxide/pharmacology Triglycerides/biosynthesis,genetics
Chemicals
Triglycerides Nitric Oxide Diacylglycerol O-Acyltransferase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sirakova Tatiana D
Biomolecular Science Center, and Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, FL 32816-2364, USA.
Dubey Vinod S
Biomolecular Science Center, and Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, FL 32816-2364, USA.
Deb Chirajyoti
Biomolecular Science Center, and Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, FL 32816-2364, USA.
Daniel Jaiyanth
Biomolecular Science Center, and Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, FL 32816-2364, USA.
Korotkova Tatiana A
Biomolecular Science Center, and Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, FL 32816-2364, USA.
Abomoelak Bassam
Biomolecular Science Center, and Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, FL 32816-2364, USA.
Kolattukudy Pappachan E
Biomolecular Science Center, and Department of Molecular Biology and Microbiology, Burnett College of Biomedical Sciences, University of Central Florida, Orlando, FL 32816-2364, USA.
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Article Info
Journal
Microbiology (Reading, England)
Abbr.
Microbiology (Reading)
ISSN
1350-0872
DOI
10.1099/mic.0.28993-0
Published
2006-09-00
Pages
2717-2725
Language
English
Region
England
NLM ID
9430468
PMCID
PMC1575465
Subset
IM
Grants
NIAID NIH HHS · R01 AI035272 · United States
NIAID NIH HHS · R01 AI046582 · United States
NIAID NIH HHS · AI35272 · United States
NIAID NIH HHS · AI46582 · United States
External Links
PubMed source DOI: 10.1099/mic.0.28993-0 PMC Full Text View journal details
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