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PMID: 1694860 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Fatty acid chain is a critical epitope for antiphospholipid antibody.

Journal of clinical immunology ·Vol. 10 ·No. 3 ·1990-05-00 ·Pages 141-5

Levy RA, Gharavi AE, Sammaritano LR, Habina L, Lockshin MD

Abstract

To explore the role of phospholipid fatty acids in binding of antiphospholipid antibody (aPL) in ELISA, we tested aPL binding to phospholipids containing fatty acids of varying chain length and degree of saturation using direct ELISA and inhibition methods. Polyclonal IgG and IgM human aPL's bind to C18:1 phosphatidylglycerol (PG) better than to C18:0 PG or C18:2 PG. Binding is greater to C18 than to C14:0 or C16:0 PGs; aPL's do not bind to C12:0 PG. aPL binding is not inhibited by C18:1 diacylglycerol, glycerol-3-phosphate, myoinositol, or myoinositol phosphate. The fatty acid chains are critical determinants for antigen recognition and, by projection, biological activity of aPL.

MeSH Terms
Autoantibodies/immunology Binding, Competitive Cardiolipins/immunology Enzyme-Linked Immunosorbent Assay Epitopes/immunology Fatty Acids/immunology Humans Phospholipids/immunology
Chemicals
Autoantibodies Cardiolipins Epitopes Fatty Acids Phospholipids
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Levy R A
Division of Rheumatic Diseases, Hospital for Special Surgery, New York, New York 10021.
Gharavi A E
Sammaritano L R
Habina L
Lockshin M D
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17 references, click to expand
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Article Info
Journal
Journal of clinical immunology
Abbr.
J Clin Immunol
ISSN
0271-9142
DOI
10.1007/BF00917913
Published
1990-05-00
Pages
141-5
Language
English
Region
Netherlands
NLM ID
8102137
Subset
IM
Grants
NIAMS NIH HHS · AR 32939 · United States
External Links
PubMed source DOI: 10.1007/BF00917913 View journal details
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