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PMID: 16950841 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Microenvironment and effect of energy depletion in the nucleus analyzed by mobility of multiple oligomeric EGFPs.

Biophysical journal ·Vol. 91 ·No. 10 ·2006-11-15 ·Pages 3921-36

Pack C, Saito K, Tamura M, Kinjo M

Abstract

Four different tandem EGFPs were constructed to elucidate the nuclear microenvironment by quantifying its diffusional properties in both aqueous solution and the nuclei of living cells. Diffusion of tandem EGFP was dependent on the length of the protein as a rod-like molecule or molecular ruler in solution. On the other hand, we found two kinds of mobility, fast diffusional mobility and much slower diffusional mobility depending on cellular compartments in living cells. Diffusion in the cytoplasm and the nucleoplasm was mainly measured as fast diffusional mobility. In contrast, diffusion in the nucleolus was complex and mainly much slower diffusional mobility, although both the fast and the slow diffusional mobilities were dependent on the protein length. Interestingly, we found that diffusion in the nucleolus was clearly changed by energy depletion, even though the diffusion in the cytoplasm and the nucleoplasm was not changed. Our results suggest that the nucleolar microenvironment is sensitive to energy depletion and very different from the nucleoplasm.

MeSH Terms
Cell Line Cell Nucleus/physiology Dimerization Energy Transfer/physiology Green Fluorescent Proteins/genetics,metabolism Humans Kidney/physiology Nuclear Proteins/metabolism Protein Transport/physiology
Chemicals
Nuclear Proteins enhanced green fluorescent protein Green Fluorescent Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pack Changi
Laboratory of Supramolecular Biophysics, Research Institute for Electronic Science, Hokkaido University, Sapporo 060-0812, Japan.
Saito Kenta
Tamura Mamoru
Kinjo Masataka
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1529/biophysj.105.079467
Published
2006-11-15
Epub
2006-00-01
Pages
3921-36
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1630477
Subset
IM
External Links
PubMed source DOI: 10.1529/biophysj.105.079467 PMC Full Text View journal details
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