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PMID: 16957775 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

bZIP10-LSD1 antagonism modulates basal defense and cell death in Arabidopsis following infection.

The EMBO journal ·Vol. 25 ·No. 18 ·2006-09-20 ·Pages 4400-11

Kaminaka H, Näke C, Epple P, Dittgen J, Schütze K, Chaban C, Holt BF, Merkle T, Schäfer E, Harter K, Dangl JL

Abstract

Plants use sophisticated strategies to balance responses to oxidative stress. Programmed cell death, including the hypersensitive response (HR) associated with successful pathogen recognition, is one cellular response regulated by reactive oxygen in various cellular contexts. The Arabidopsis basic leucine zipper (bZIP) transcription factor AtbZIP10 shuttles between the nucleus and the cytoplasm and binds consensus G- and C-box DNA sequences. Surprisingly, AtbZIP10 can be retained outside the nucleus by LSD1, a protein that protects Arabidopsis cells from death in the face of oxidative stress signals. We demonstrate that AtbZIP10 is a positive mediator of the uncontrolled cell death observed in lsd1 mutants. AtbZIP10 and LSD1 act antagonistically in both pathogen-induced HR and basal defense responses. LSD1 likely functions as a cellular hub, where its interaction with AtbZIP10 and additional, as yet unidentified, proteins contributes significantly to plant oxidative stress responses.

MeSH Terms
Apoptosis Arabidopsis/cytology,genetics,metabolism Arabidopsis Proteins/genetics,metabolism Basic-Leucine Zipper Transcription Factors/genetics,metabolism Cytoplasm/metabolism DNA-Binding Proteins/genetics,metabolism Genes, Plant Green Fluorescent Proteins/genetics,metabolism Mutation Oomycetes/pathogenicity Oxidative Stress Plant Diseases/genetics,microbiology Plants, Genetically Modified Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Transcription Factors/genetics,metabolism Two-Hybrid System Techniques
Chemicals
Arabidopsis Proteins Basic-Leucine Zipper Transcription Factors DNA-Binding Proteins LSD1 protein, Arabidopsis Recombinant Fusion Proteins Transcription Factors Green Fluorescent Proteins
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Kaminaka Hironori
Department of Biology, Curriculum in Genetics and Carolina Center for Genome Sciences, CB#3280, University of North Carolina, Chapel Hill, NC 27599, USA.
Näke Christian
Epple Petra
Dittgen Jan
Schütze Katia
Chaban Christina
Holt Ben F
Merkle Thomas
Schäfer Eberhard
Harter Klaus
Dangl Jeffery L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7601312
Published
2006-09-20
Epub
2006-00-07
Pages
4400-11
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1570446
Subset
IM
Grants
NIGMS NIH HHS · R01 GM057171 · United States
NIGMS NIH HHS · R01-GM057171 · United States
External Links
PubMed source DOI: 10.1038/sj.emboj.7601312 PMC Full Text View journal details
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