Home LiteratureArticle Details
PMID: 1696953 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunological crossreactivity between the human immunodeficiency virus type 1 virion infectivity factor and a 170-kD surface antigen of Schistosoma mansoni.

The Journal of experimental medicine ·Vol. 172 ·No. 3 ·1990-09-01 ·Pages 1001-4

Khalife J, Grzych JM, Pierce R, Ameisen JC, Schacht AM, Gras-Masse H, Tartar A, Lecocq JP, Capron A

Abstract

A monoclonal antibody (mAb) directed against a synthetic peptide derived from the sequence of the human immunodeficiency virus type 1 (HIV-1) regulatory protein virion infectivity factor (vif) labeled the surface of Schistosoma mansoni schistosomula by indirect immunofluorescence. Western blotting showed that two S. mansoni proteins of 170 and 65 kD were recognized by the mAb. Sera from 20% of S. mansoni-infected HIV-seronegative individuals tested recognized the PS4 peptide in an ELISA as did sera from S. mansoni-infected rats. Sera from individuals seropositive for HIV-1, but without schistosomiasis, that reacted with the vif peptide also recognized a 170-kD S. mansoni protein. This crossreactive S. mansoni antigen appears to be a target of immunity in vivo since passive transfer of the mAb VIF-CD3 to naive rats had a protective effect against a challenge infection with S. mansoni cercariae.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal Antigens, Helminth/immunology Antigens, Surface/immunology Child Cross Reactions Enzyme-Linked Immunosorbent Assay Epitopes/analysis Fluorescent Antibody Technique Gene Products, vif HIV Seropositivity HIV-1/immunology Humans Molecular Sequence Data Peptides/chemical synthesis Rats Rats, Inbred Strains Schistosoma mansoni/immunology Schistosomiasis mansoni/immunology Viral Regulatory and Accessory Proteins/immunology Virion/immunology vif Gene Products, Human Immunodeficiency Virus
Chemicals
Antibodies, Monoclonal Antigens, Helminth Antigens, Surface Epitopes Gene Products, vif Peptides Viral Regulatory and Accessory Proteins vif Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Khalife J
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM, U167-CNRS 624, Strasbourg, France.
Grzych J M
Pierce R
Ameisen J C
Schacht A M
Gras-Masse H
Tartar A
Lecocq J P
Capron A
References (14)
14 references, click to expand
  1. Isotypic restriction of the antibody response to human immunodeficiency virus.
    AIDS Res Hum Retroviruses. 1988 Feb;4(1):3-9 PMID: 3163253
  2. HIV F/3' orf encodes a phosphorylated GTP-binding protein resembling an oncogene product.
    Nature. 1987 Nov 19-25;330(6145):266-9 PMID: 3118220
  3. ELISA HTLV retrovirus antibody reactivity associated with malaria and immune complexes in healthy Africans.
    Lancet. 1985 Sep 7;2(8454):520-3 PMID: 2863543
  4. Regional variation in prevalence of antibody against human T-lymphotropic virus types I and III in Kenya, East Africa.
    Int J Cancer. 1985 Jun 15;35(6):763-7 PMID: 2989191
  5. Replicative and cytopathic potential of HTLV-III/LAV with sor gene deletions.
    Science. 1986 Mar 28;231(4745):1549-53 PMID: 3006244
  6. Absence of cross-reactivity between Schistosoma mansoni infection and human immunodeficiency virus (HIV).
    Trans R Soc Trop Med Hyg. 1988;82(2):262 PMID: 3142117
  7. The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms.
    Parasitology. 1965 Nov;55(4):695-700 PMID: 4957633
  8. Antibodies to the nef protein and to nef peptides in HIV-1-infected seronegative individuals.
    AIDS Res Hum Retroviruses. 1989 Jun;5(3):279-91 PMID: 2786420
  9. Functional role of human IgG subclasses in eosinophil-mediated killing of schistosomula of Schistosoma mansoni.
    J Immunol. 1989 Jun 15;142(12):4422-7 PMID: 2723436
  10. Immunity after treatment of human schistosomiasis mansoni. II. Identification of resistant individuals, and analysis of their immune responses.
    Trans R Soc Trop Med Hyg. 1985;79(3):393-408 PMID: 4035741
  11. Immunity after treatment of human schistosomiasis mansoni. I. Study design, pretreatment observations and the results of treatment.
    Trans R Soc Trop Med Hyg. 1984;78(1):108-23 PMID: 6710563
  12. In vitro and in vivo effector function of rat IgG2a monoclonal anti-S. mansoni antibodies.
    J Immunol. 1982 Dec;129(6):2739-43 PMID: 7142705
  13. The effects of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro.
    Int J Parasitol. 1972 Mar;2(1):79-98 PMID: 4631607
  14. Immunity in human schistosomiasis mansoni. Regulation of protective immune mechanisms by IgM blocking antibodies.
    J Exp Med. 1986 Nov 1;164(5):1626-40 PMID: 2430044
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.172.3.1001
Published
1990-09-01
Pages
1001-4
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188553
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.172.3.1001 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2