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PMID: 1697030 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a cDNA encoding a cysteine-rich cell surface protein located in the flagellar pocket of the protozoan Trypanosoma brucei.

Molecular and cellular biology ·Vol. 10 ·No. 9 ·1990-09-00 ·Pages 4506-17

Lee MG, Bihain BE, Russell DG, Deckelbaum RJ, Van der Ploeg LH

Abstract

We have characterized a cDNA encoding a cysteine-rich, acidic integral membrane protein (CRAM) of the parasitic protozoa Trypanosoma brucei and Trypanosoma equiperdum. Unlike other membrane proteins of T. brucei, which are distributed throughout the cell surface, CRAM is concentrated in the flagellar pocket, an invagination of the cell surface of the trypanosome where endocytosis has been documented. Accordingly, CRAM also locates to vesicles located underneath the pocket, providing evidence of its internalization. CRAM has a predicted molecular mass of 130 kilodaltons and has a signal peptide, a transmembrane domain, and a 41-amino-acid cytoplasmic extension. A characteristic feature of CRAM is a large extracellular domain with a roughly 66-fold acidic, cysteine-rich 12-amino-acid repeat. CRAM is conserved among different protozoan species, including Trypanosoma cruzi, and CRAM has structural similarities with eucaryotic cell surface receptors. The most striking homology of CRAM is to the human low-density-lipoprotein receptor. We propose that CRAM functions as a cell surface receptor of different trypanosome species.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/genetics,isolation & purification Escherichia coli/genetics Immunoblotting Male Membrane Proteins/analysis,genetics Microscopy, Electron Molecular Sequence Data Protein Conformation Protozoan Proteins/analysis,genetics RNA/genetics,isolation & purification Rats Rats, Inbred Strains Restriction Mapping Sequence Homology, Nucleic Acid Trypanosoma brucei brucei/genetics,ultrastructure
Chemicals
Membrane Proteins Protozoan Proteins cysteine-rich, acidic integral membrane protein, Trypanosoma brucei RNA DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lee M G
Department of Genetics and Development, Columbia University, New York, New York 10032.
Bihain B E
Russell D G
Deckelbaum R J
Van der Ploeg L H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.9.4506-4517.1990
Published
1990-09-00
Pages
4506-17
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361037
Subset
IM
Grants
NIAID NIH HHS · AI 21784 · United States
NHLBI NIH HHS · HL 21006 · United States
Databases
GENBANK
M55686
External Links
PubMed source DOI: 10.1128/mcb.10.9.4506-4517.1990 PMC Full Text View journal details
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