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PMID: 16973580 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cellular target genes of Epstein-Barr virus nuclear antigen 2.

Journal of virology ·Vol. 80 ·No. 19 ·2006-10-00 ·Pages 9761-71

Maier S, Staffler G, Hartmann A, Höck J, Henning K, Grabusic K, Mailhammer R, Hoffmann R, Wilmanns M, Lang R, Mages J, Kempkes B

Abstract

Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA-2) is a key determinant in the EBV-driven B-cell growth transformation process. By activating an array of viral and cellular target genes, EBNA-2 initiates a cascade of events which ultimately cause cell cycle entry and the proliferation of the infected B cell. In order to identify cellular target genes that respond to EBNA-2 in the absence of other viral factors, we have performed a comprehensive search for EBNA-2 target genes in two EBV-negative B-cell lines. This screen identified 311 EBNA-2-induced and 239 EBNA-2-repressed genes that were significantly regulated in either one or both cell lines. The activation of most of these genes had not previously been attributed to EBNA-2 function and will be relevant for the identification of EBNA-2-specific contributions to EBV-associated malignancies. The diverse spectrum of EBNA-2 target genes described in this study reflects the broad spectrum of EBNA-2 functions involved in virus-host interactions, including cell signaling molecules, adapters, genes involved in cell cycle regulation, and chemokines.

MeSH Terms
B-Lymphocytes/metabolism Cell Line DNA-Binding Proteins/genetics,metabolism Down-Regulation/genetics Epstein-Barr Virus Nuclear Antigens/genetics,metabolism Herpesvirus 4, Human/genetics,metabolism Humans Oligonucleotide Array Sequence Analysis RNA, Messenger/genetics Receptors, Notch/genetics,metabolism
Chemicals
DNA-Binding Proteins Epstein-Barr Virus Nuclear Antigens RNA, Messenger Receptors, Notch
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Maier Sabine
GSF-National Research Center for Environment and Health, Institute of Clinical Molecular Biology, Munich, Germany.
Staffler Gabriele
Hartmann Andrea
Höck Julia
Henning Karen
Grabusic Kristina
Mailhammer Reinhard
Hoffmann Reinhard
Wilmanns Matthias
Lang Roland
Mages Jörg
Kempkes Bettina
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.00665-06
Published
2006-10-00
Pages
9761-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC1617228
Subset
IM
Databases
GEO
GSE4525
External Links
PubMed source DOI: 10.1128/JVI.00665-06 PMC Full Text View journal details
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