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PMID: 1697945 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Activation of subconductance states by gamma-aminobutyric acid and its analogs in chick cerebral neurons.

Pflugers Archiv : European journal of physiology ·Vol. 416 ·No. 4 ·1990-06-00 ·Pages 454-61

Mistry DK, Hablitz JJ

Abstract

The action of gamma-aminobutyric acid (GABA) and the GABAA-receptor agonists muscimol, isoguvacine and 4,5,6,7-tetrahydroisoxazolo [5,4-c]pyridin-3-ol (THIP) were studied at the single-channel level in outside-out membrane patches from cultured chick cerebral neurons. All agonists activated channels with multiple-conductance states. The main-state conductance activated by all agonists had a value around 26 pS in symmetrical TRIS/Cl solutions. Subconductance states of around 13 pS and 18 pS were seen with application of each agonist. Muscimol and isoguvacine tended preferentially to activate subconductance states. Gating by all agonists was complex. Open-time distributions for main-state activity gated by GABA, isoguvacine and THIP were best described by the sum of two exponential curves with similar time constants. Muscimol-gated activity was best described by the sum of three exponentials indicating the presence of an additional longer open state. These results indicate that certain GABAA-receptor agonists are capable of preferentially activating subconductance states.

MeSH Terms
Analgesics/pharmacology Animals Cells, Cultured Cerebral Cortex/cytology,drug effects,physiology Chick Embryo Chlorides Electric Conductivity/drug effects,physiology Ion Channel Gating/drug effects,physiology Ion Channels/metabolism,physiology,ultrastructure Isonicotinic Acids/pharmacology Isoxazoles/pharmacology Muscimol/pharmacology Neurons/drug effects,physiology,ultrastructure gamma-Aminobutyric Acid/pharmacology
Chemicals
Analgesics Chlorides Ion Channels Isonicotinic Acids Isoxazoles Muscimol gamma-Aminobutyric Acid gaboxadol isoguvacine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mistry D K
Neurobiology Research Center, University of Alabama, Birmingham 35294.
Hablitz J J
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00370754
Published
1990-06-00
Pages
454-61
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
Grants
NINDS NIH HHS · NS11535 · United States
NINDS NIH HHS · NS18145 · United States
External Links
PubMed source DOI: 10.1007/BF00370754 View journal details
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