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PMID: 16997946 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Global map of growth-regulated gene expression in Burkholderia pseudomallei, the causative agent of melioidosis.

Journal of bacteriology ·Vol. 188 ·No. 23 ·2006-12-00 ·Pages 8178-88

Rodrigues F, Sarkar-Tyson M, Harding SV, Sim SH, Chua HH, Lin CH, Han X, Karuturi RK, Sung K, Yu K, Chen W, Atkins TP, Titball RW, Tan P

Abstract

Many microbial pathogens express specific virulence traits at distinct growth phases. To understand the molecular pathways linking bacterial growth to pathogenicity, we have characterized the growth transcriptome of Burkholderia pseudomallei, the causative agent of melioidosis. Using a fine-scale sampling approach, we found approximately 17% of all B. pseudomallei genes displaying regulated expression during growth in rich medium, occurring as broad waves of functionally coherent gene expression tightly associated with distinct growth phases and transition points. We observed regulation of virulence genes across all growth phases and identified serC as a potentially new virulence factor by virtue of its coexpression with other early-phase virulence genes. serC-disrupted B. pseudomallei strains were serine auxotrophs and in mouse infection assays exhibited a dramatic attenuation of virulence compared to wild-type B. pseudomallei. Immunization of mice with serC-disrupted B. pseudomallei also conferred protection against subsequent challenges with different wild-type B. pseudomallei strains. At a genomic level, early-phase genes were preferentially localized on chromosome 1, while stationary-phase genes were significantly biased towards chromosome 2. We detected a significant level of chromosomally clustered gene expression, allowing us to predict approximately 100 potential operons in the B. pseudomallei genome. We computationally and experimentally validated these operons by showing that genes in these regions are preferentially transcribed in the same 5'-->3' direction, possess significantly shorter intergenic lengths than the overall genome, and are expressed as a common mRNA transcript. The availability of this transcriptome map provides an important resource for understanding the transcriptional architecture of B. pseudomallei.

MeSH Terms
Animals Bacterial Vaccines/administration & dosage Burkholderia pseudomallei/genetics,growth & development,immunology,pathogenicity Chromosomes, Bacterial/genetics Female Gene Deletion Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Bacterial Genome, Bacterial Melioidosis/microbiology,prevention & control Mice Mice, Inbred BALB C Multigene Family Oligonucleotide Array Sequence Analysis Vaccination Virulence Virulence Factors/genetics
Chemicals
Bacterial Vaccines Virulence Factors
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Rodrigues Fiona
Genome Institute of Singapore, 60 Biopolis Street, no. 02-01, Genome, Singapore 138672, Republic of Singapore.
Sarkar-Tyson Mitali
Harding Sarah V
Sim Siew Hoon
Chua Hui Hoon
Lin Chi Ho
Han Xu
Karuturi R Krishna M
Sung Ken
Yu Kun
Chen Wei
Atkins Timothy P
Titball Richard W
Tan Patrick
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.01006-06
Published
2006-12-00
Epub
2006-00-22
Pages
8178-88
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1698202
Subset
IM
Databases
GEO
GSE5495
External Links
PubMed source DOI: 10.1128/JB.01006-06 PMC Full Text View journal details
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