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PMID: 17007026 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Assessment of oxidative stress in chronic pancreatitis patients.

World journal of gastroenterology ·Vol. 12 ·No. 35 ·2006-09-21 ·Pages 5705-10

Verlaan M, Roelofs HM, van-Schaik A, Wanten GJ, Jansen JB, Peters WH, Drenth JP

Abstract

To assess the levels of antioxidant capacity and oxidative damage in blood of chronic pancreatitis (CP) patients in comparison with those in healthy control subjects, by using several different analytical techniques. Thirty-five CP patients and 35 healthy control subjects were investigated prospectively with respect to plasma levels of thiols, ferric reducing ability of plasma (FRAP, i.e. antioxidant capacity), levels of protein carbonyls and thiobarbituric acid reactive substances (TBARS). Additionally, we evaluated the production of reactive oxygen species (ROS) in whole blood. The antioxidative thiols including cysteine, cysteinylglycine and glutathione were significantly lower in CP patients. In addition, the non-enzymatic antioxidant capacity was significantly lower in CP patients, which correlated with the amount of oxidative protein (protein carbonyls) and the extent of lipid damage (TBARS), both were significantly higher in CP patients. The ROS production in whole blood after stimulation with phorbol 12-myritate 13-acetaat, demonstrated a strong tendency to produce more ROS in CP patients. Oxidative stress may contribute to the pathogenesis of chronic pancreatitis by decreasing antioxidant capacity and increasing oxidative damage in CP patients may be a rationale for intervention with antioxidant therapy.

MeSH Terms
Adult Aged Antioxidants/pharmacology,therapeutic use Cysteine/blood Female Glutathione/blood Humans Lipid Peroxidation/physiology Male Middle Aged Oxidative Stress/drug effects,physiology Pancreatitis, Chronic/blood,drug therapy,physiopathology Prospective Studies Protein Carbonylation/physiology Reactive Oxygen Species/blood Sulfhydryl Compounds/blood Thiobarbituric Acid Reactive Substances/analysis
Chemicals
Antioxidants Reactive Oxygen Species Sulfhydryl Compounds Thiobarbituric Acid Reactive Substances Glutathione Cysteine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Verlaan Mariette
Department of Gastroenterology, Radboud University Nijmegen Medical Centre, PO Box 9101, Nijmegen 6500 HB, The Netherlands. [email protected]
Roelofs Hennie M J
van-Schaik Annie
Wanten Geert J A
Jansen Jan B M J
Peters Wilbert H M
Drenth Joost P H
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Article Info
Journal
World journal of gastroenterology
Abbr.
World J Gastroenterol
ISSN
1007-9327
DOI
10.3748/wjg.v12.i35.5705
Email
[email protected]
Published
2006-09-21
Pages
5705-10
Language
English
Region
United States
NLM ID
100883448
PMCID
PMC4088174
Subset
IM
External Links
PubMed source DOI: 10.3748/wjg.v12.i35.5705 PMC Full Text View journal details
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