Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using a monoclonal antibody.

Journal of clinical microbiology ·Vol. 28 ·No. 11 ·1990-11-00 ·Pages 2409-14

Thorns CJ, Sojka MG, Chasey D

Abstract

A fimbrialike structure expressed on the surface of Salmonella enteritidis was identified by using a monoclonal antibody (69/25) produced against intact S. enteritidis cells. Fimbriae were less than 5 nm in diameter and carried a protein consisting of subunits with a molecular weight of 14,300. No hemagglutinating activity associated with the fimbriae was detected. An epitope on the fimbrial antigen identified by monoclonal antibody 69/25 was expressed by all 58 S. enteritidis strains, 12 of 36 S. dublin strains, and a single strain of S. moscow examined. None of 169 other isolates tested from 17 salmonella serogroups expressed this epitope.

MeSH Terms

Antibodies, Monoclonal Antigens, Bacterial Antigens, Surface Epitopes Fimbriae, Bacterial/immunology,ultrastructure Hemagglutination Tests Microscopy, Electron Salmonella enteritidis/classification,immunology,ultrastructure Serotyping

Chemicals

Antibodies, Monoclonal Antigens, Bacterial Antigens, Surface Epitopes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Thorns C J

Central Veterinary Laboratory, Ministry of Agriculture, Fisheries and Food, Weybridge, Surrey, United Kingdom.

Sojka M G

Chasey D

References (13)

13 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.28.11.2409-2414.1990

Published

1990-11-00

Pages

2409-14

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC268197

Subset

External Links

PubMed source DOI: 10.1128/jcm.28.11.2409-2414.1990 PMC Full Text View journal details

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Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using a monoclonal antibody.

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