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PMID: 1701821 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Structure-function relationships among highly diverse T cells that recognize a determinant from influenza virus hemagglutinin.

The Journal of experimental medicine ·Vol. 172 ·No. 6 ·1990-12-01 ·Pages 1643-51

Taylor AH, Haberman AM, Gerhard W, Caton AJ

Abstract

We have analyzed the structural and genetic basis for T cell recognition of the complex formed between antigen and class II products of the major histocompatibility complex by performing sequence analysis of T cell receptors (TCRs) induced in response to the helper T cell site 1 of the influenza virus hemagglutinin. The results demonstrate, first, that structurally highly diverse TCRs can be utilized in recognition of the same antigen/I-Ed complex: 12 of 13 TCRs utilize unique V alpha/V beta gene segment combinations, suggesting that approximately 70 different V alpha/V beta combinations are available to BALB/c mice in response to this determinant. Second, comparison of these sequences with the ability of each hybridoma to recognize a panel of peptide analogues suggests that alpha and beta chains of these TCRs frequently determine specificity for the NH2-terminal and the COOH terminal portions, respectively, of the site 1 determinant.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Epitopes/immunology Genes, MHC Class II Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral/immunology Macromolecular Substances Mice Mice, Inbred BALB C Molecular Sequence Data Oligonucleotide Probes Receptors, Antigen, T-Cell/genetics,immunology Sequence Homology, Nucleic Acid T-Lymphocytes/immunology T-Lymphocytes, Helper-Inducer/immunology
Chemicals
Epitopes Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral Macromolecular Substances Oligonucleotide Probes Receptors, Antigen, T-Cell
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Taylor A H
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylyvania 19104.
Haberman A M
Gerhard W
Caton A J
References (35)
35 references, click to expand
  1. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
    Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
  2. On the thymus in the differentiation of "H-2 self-recognition" by T cells: evidence for dual recognition?
    J Exp Med. 1978 Mar 1;147(3):882-96 PMID: 305459
  3. Restricted use of T cell receptor V genes in murine autoimmune encephalomyelitis raises possibilities for antibody therapy.
    Cell. 1988 Aug 12;54(4):577-92 PMID: 2456857
  4. One-sided polymerase chain reaction: the amplification of cDNA.
    Proc Natl Acad Sci U S A. 1989 Aug;86(15):5673-7 PMID: 2788276
  5. Use of primer-restriction-end adapters in a novel cDNA cloning strategy.
    Gene. 1985;34(2-3):305-14 PMID: 2408965
  6. T cell receptor gene usage in the response to lambda repressor cI protein. An apparent bias in the usage of a V alpha gene element.
    J Exp Med. 1988 Sep 1;168(3):1081-97 PMID: 2971753
  7. Structural and functional implications of a restricted antibody response to a defined antigenic region on the influenza virus hemagglutinin.
    EMBO J. 1986 Jul;5(7):1577-87 PMID: 2427335
  8. A synthetic decapeptide of influenza virus hemagglutinin elicits helper T cells with the same fine recognition specificities as occur in response to whole virus.
    J Immunol. 1985 Aug;135(2):1391-4 PMID: 2409149
  9. Selection of amino acid sequences in the beta chain of the T cell antigen receptor.
    Science. 1988 Mar 25;239(4847):1541-4 PMID: 2832942
  10. Generation of diversity in T cell receptor repertoire specific for pigeon cytochrome c.
    J Exp Med. 1987 Feb 1;165(2):279-301 PMID: 3029266
  11. Selection of variable-joining region combinations in the alpha chain of the T cell receptor.
    Science. 1988 Sep 9;241(4871):1354-8 PMID: 2970673
  12. Molecular analysis of the antigen receptor of virus-specific cytotoxic T cells and identification of a new V alpha family.
    Eur J Immunol. 1987 Dec;17(12):1843-6 PMID: 2961577
  13. T-cell antigen receptor genes and T-cell recognition.
    Nature. 1988 Aug 4;334(6181):395-402 PMID: 3043226
  14. Site-directed mutations in the VDJ junctional region of a T cell receptor beta chain cause changes in antigenic peptide recognition.
    Cell. 1988 Aug 12;54(4):473-84 PMID: 2456856
  15. Both rat and mouse T cell receptors specific for the encephalitogenic determinant of myelin basic protein use similar V alpha and V beta chain genes even though the major histocompatibility complex and encephalitogenic determinants being recognized are different.
    J Exp Med. 1989 Jan 1;169(1):27-39 PMID: 2462609
  16. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002 PMID: 2461560
  17. Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain.
    Science. 1989 Jan 13;243(4888):217-20 PMID: 2463672
  18. The T cell receptor: the alpha and beta chains define idiotype, and antigen and MHC specificity.
    Cell. 1985 Aug;42(1):81-7 PMID: 2410143
  19. T-lymphocyte recognition of antigen in association with gene products of the major histocompatibility complex.
    Annu Rev Immunol. 1985;3:237-61 PMID: 2415139
  20. A hypothetical model of the foreign antigen binding site of class II histocompatibility molecules.
    Nature. 1988 Apr 28;332(6167):845-50 PMID: 3258651
  21. The T cell receptor V alpha 3 gene segment is associated with reactivity to p-azobenzenearsonate.
    Cell. 1988 Jul 15;54(2):247-61 PMID: 3260539
  22. Characterization of the murine TH response to influenza virus hemagglutinin: evidence for three major specificities.
    J Immunol. 1984 Dec;133(6):3371-7 PMID: 6208279
  23. Dominance of one T-cell receptor in the H-2Kb/TNP response.
    Nature. 1987 Mar 19-25;326(6110):307-9 PMID: 3493439
  24. T cell clones specific for an amphipathic alpha-helical region of sperm whale myoglobin show differing fine specificities for synthetic peptides. A multiview/single structure interpretation of immunodominance.
    J Exp Med. 1986 Nov 1;164(5):1779-84 PMID: 3490536
  25. In a radiation chimaera, host H-2 antigens determine immune responsiveness of donor cytotoxic cells.
    Nature. 1977 Sep 29;269(5627):417-8 PMID: 302918
  26. Correlation of T cell receptor V beta gene family with MHC restriction.
    J Exp Med. 1987 Aug 1;166(2):583-8 PMID: 3598467
  27. A set of closely related antibodies dominates the primary antibody response to the antigenic site CB of the A/PR/8/34 influenza virus hemagglutinin.
    J Immunol. 1990 Oct 1;145(7):2312-21 PMID: 2118933
  28. T cell receptor junctional regions and the MHC molecule affect the recognition of antigenic peptides by T cell clones.
    J Immunol. 1990 Feb 1;144(3):1127-35 PMID: 1688589
  29. The recognition specificity of a murine helper T cell for hemagglutinin of influenza virus A/PR/8/34.
    J Immunol. 1983 May;130(5):2379-85 PMID: 6187854
  30. Influenza virus site recognized by a murine helper T cell specific for H1 strains. Localization to a nine amino acid sequence in the hemagglutinin molecule.
    J Exp Med. 1983 Aug 1;158(2):294-302 PMID: 6224879
  31. The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype).
    Cell. 1982 Dec;31(2 Pt 1):417-27 PMID: 6186384
  32. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
    J Immunol Methods. 1983 Dec 16;65(1-2):55-63 PMID: 6606682
  33. Genomic organization and sequence of T-cell receptor beta-chain constant- and joining-region genes.
    Nature. 1984 Aug 2-8;310(5976):387-91 PMID: 6336329
  34. Mouse T cell antigen receptor: structure and organization of constant and joining gene segments encoding the beta polypeptide.
    Cell. 1984 Jul;37(3):1101-10 PMID: 6611207
  35. Limited heterogeneity of T cell receptors from lymphocytes mediating autoimmune encephalomyelitis allows specific immune intervention.
    Cell. 1988 Jul 15;54(2):263-73 PMID: 2455603
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.172.6.1643
Published
1990-12-01
Pages
1643-51
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188746
Subset
IM
Grants
NIAID NIH HHS · AI-13989 · United States
NIAID NIH HHS · AI-24541 · United States
Databases
GENBANK
M34194, M34219
External Links
PubMed source DOI: 10.1084/jem.172.6.1643 PMC Full Text View journal details
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