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PMID: 170207 Published · ppublish English Journal Article

Increased adhesion of Chinese hamster ovary cells to substratum by cholera enterotoxin.

Infection and immunity ·Vol. 12 ·No. 3 ·1975-09-00 ·Pages 621-4

Nozawa R, Kon F, Yokota T, Ohashi M, Kuwahara S

Abstract

A new, simple assay method using Chinese hamster ovary cells was devised for cholera enterotoxin. The effect of the cholera toxin was measured by an increase in cell adhesion to the substratum. The increase was dependent on the concentration of the toxin used and was effective for a longer period of time than that of adhesion increased by dibutyryl cyclic adenosine monophosphate and theophyline. There was a delay of about 60 min before the increase in adhesion, using the toxin, appeared, whereas the increase caused by dibutyryl cyclic adenosine monophosphate appeared almost at once.

MeSH Terms
Animals Bucladesine Cell Adhesion Cell Count Cholera/immunology Cricetinae Culture Techniques Enterotoxins Female Microscopy, Phase-Contrast Ovary/immunology
Chemicals
Enterotoxins Bucladesine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nozawa R
Kon F
Yokota T
Ohashi M
Kuwahara S
References (22)
22 references, click to expand
  1. CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.
    Proc Natl Acad Sci U S A. 1965 Feb;53:288-93 PMID: 14294058
  2. Preparation of the vascular permeability factor of Vibrio cholerae.
    J Bacteriol. 1966 Sep;92(3):793-5 PMID: 5922548
  3. Stimulation of intestinal adenyl cyclase by cholera toxin.
    Nature. 1971 Jan 22;229(5282):266-9 PMID: 4323551
  4. Stimulation of intestinal mucosal adenyl cyclase by cholera enterotoxin and prostaglandins.
    J Clin Invest. 1971 Jun;50(6):1218-30 PMID: 4325309
  5. Elevated concentration of adenosine 3':5'-cyclic monophosphate in intestinal mucosa after treatment with cholera toxin.
    Proc Natl Acad Sci U S A. 1970 Oct;67(2):851-6 PMID: 4331724
  6. Membrane dynamics in the action of dibutyryl adenosine 3':5'-cyclic monophosphate and testosterone on mammalian cells.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1943-7 PMID: 4340166
  7. Induction of steroidogenesis in tissue culture by cholera enterotoxin.
    Nat New Biol. 1973 Jun 20;243(129):246-7 PMID: 4351987
  8. Pleiotypic control by cyclic AMP: interaction with cyclic GMP and possible role of microtubules.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1659-63 PMID: 4352647
  9. Stimulation of steroid secretion in adrenal tumor cells by choleragen.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2741-4 PMID: 4355364
  10. Epidermal growth factor: receptors in human fibroblasts and modulation of action by cholera toxin.
    Proc Natl Acad Sci U S A. 1973 Oct;70(10):2964-8 PMID: 4355377
  11. Cholera toxin mimics melanocyte stimulating hormone in inducing differentiation in melanoma cells.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2500-4 PMID: 4366771
  12. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.
    Infect Immun. 1974 Aug;10(2):320-7 PMID: 4368545
  13. Cholera toxin inhibits macromolecular synthesis in mouse spleen cells.
    Nat New Biol. 1973 Aug 8;244(136):178-80 PMID: 4541993
  14. Professor G. Ya. Bei-Bienko.
    Nature. 1972 Mar 31;236(5344):247-8 PMID: 4553644
  15. Differential inhibitory effects of cholera toxoids and ganglioside on the enterotoxins of Vibrio cholerae and Escherichia coli.
    J Exp Med. 1973 Apr 1;137(4):1009-23 PMID: 4571325
  16. Deactivation of cholera toxin by a sialidase-resistant monosialosylganglioside.
    J Infect Dis. 1973 Jun;127(6):639-47 PMID: 4707310
  17. Gangliosides and membrane receptors for cholera toxin.
    Biochemistry. 1973 Aug 28;12(18):3558-66 PMID: 4731192
  18. Cholera toxin-fat cell interaction and the mechanism of activation of the lipolytic response.
    Biochemistry. 1973 Aug 28;12(18):3567-77 PMID: 4731193
  19. Intestinal adenyl-cyclase activity in canine cholera: correlation with fluid accumulation.
    J Infect Dis. 1972 Apr;125(4):377-81 PMID: 5018378
  20. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.
    Proc Natl Acad Sci U S A. 1968 Aug;60(4):1275-81 PMID: 5244736
  21. Effects of prostaglandins, theophylline, and cholera exotoxin upon transmucosal water and electrolyte movement in the canine jejunum.
    Gastroenterology. 1971 Jan;60(1):22-32 PMID: 5544090
  22. Stimulation of glycerol production in fat cells by cholera toxin.
    Nature. 1970 May 16;226(5246):658-9 PMID: 4315553
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.12.3.621-624.1975
Published
1975-09-00
Pages
621-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415332
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.12.3.621-624.1975 PMC Full Text View journal details
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