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PMID: 1702506 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regions of the cloned Vibrio cholerae rfb genes needed to determine the Ogawa form of the O-antigen.

Molecular & general genetics : MGG ·Vol. 224 ·No. 3 ·1990-12-00 ·Pages 405-12

Morona R, Matthews MS, Morona JK, Brown MH

Abstract

The O-antigen of the lipopolysaccharides of Vibrio cholerae 01 can exist in two forms termed Inaba and Ogawa. We used a complementation system to demonstrate that the Ogawa phenotype is dominant over the Inaba phenotype. By using a set of deletions affecting the Ogawa rfb genes, we identified two regions which are needed to confer the Ogawa phenotype. In vitro mutagenesis of the cloned Ogawa rfb genes resulted in the isolation of variants with the Inaba phenotype. The results are interpreted with respect to previous studies demonstrating interconversion between the two forms of the V. cholerae O-antigen.

Related Genes
rfb
MeSH Terms
Antigens, Bacterial/genetics Cloning, Molecular DNA Mutational Analysis DNA, Bacterial/genetics Genes, Bacterial Genes, Dominant Genetic Complementation Test Hydroxylamine Hydroxylamines/pharmacology Mutagenesis O Antigens Restriction Mapping Vibrio cholerae/genetics,immunology
Chemicals
Antigens, Bacterial DNA, Bacterial Hydroxylamines O Antigens Hydroxylamine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Morona R
Enterovax Limited, Adelaide, South Australia.
Matthews M S
Morona J K
Brown M H
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20 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00262435
Published
1990-12-00
Pages
405-12
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00262435
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