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PMID: 170420 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sulfated components of enveloped viruses.

Journal of virology ·Vol. 16 ·No. 4 ·1975-10-00 ·Pages 859-66

Pinter A, Compans RW

Abstract

The glycoproteins of several enveloped viruses, grown in a variety of cell types, are labeled with 35SO4(-2), whereas the nonglycosylated proteins are not. This was shown for the HN and F glycoproteins of SV5 and Sendai virus, the E1 and E2 glycoproteins of Sindbis virus, and for the major glycoprotein, gp69, as well as for a minor glycoprotein, gp52, of Rauscher leukemia virus. The minor glycoprotein of Rauscher leukemia virus is more highly sulfated, with a ratio of 35SO4- [3H]glucosamine about threefold greater than that of gp69. The G protein of vesicular stomatitis virus was labeled when virions were grown in the MDBK line of bovine kidney cells, although no significant incorporation of 35SO4(-2) into this protein was observed in virions grown in BHK21-F line of baby hamster kidney cells. In addition to the viral glycoproteins, sulfate was also incorporated into a heterogenous component with an electrophoretic mobility lower than that of any labeled with 35SO4(-2) and [3H]leucine, this component had a much greater 35S-3H ratio than any of the viral polypeptides and thus could not represent aggregated viral proteins. This material is believed to be a cell-derived mucopolysaccharide and can be removed from virions by treatment with hyaluronidase without affecting the amount of sulfate present on the glycoproteins.

MeSH Terms
Cell Line Glucosamine/analysis Glycoproteins/analysis Molecular Weight Parainfluenza Virus 1, Human/analysis Paramyxoviridae/analysis RNA Viruses/analysis Rauscher Virus/analysis Sindbis Virus/analysis Sulfates/analysis Viral Proteins/analysis
Chemicals
Glycoproteins Sulfates Viral Proteins Glucosamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pinter A
Compans R W
References (27)
27 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.16.4.859-866.1975
Published
1975-10-00
Pages
859-66
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354746
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.16.4.859-866.1975 PMC Full Text View journal details
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