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PMID: 1705010 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of domains of the v-crk oncogene product sufficient for association with phosphotyrosine-containing proteins.

Molecular and cellular biology ·Vol. 11 ·No. 3 ·1991-03-00 ·Pages 1607-13

Matsuda M, Mayer BJ, Hanafusa H

Abstract

The oncogene product of the avian sarcoma virus CT10, P47gag-crk, contains the SH2, SH2', and SH3 domains and binds proteins in a phosphotyrosine (ptyr)-dependent manner. In this study, we have determined the region of P47gag-crk essential for binding to ptyr-containing proteins. Mutant P47gag-crk proteins expressed in Escherichia coli that have the intact SH2 and SH2' regions retained the capacity to bind ptyr-containing proteins obtained from cells transformed by crk and src. The deletion of SH2 resulted in the loss of binding activity. Other mutants that have altered SH2 or SH2' bound few, if any, of the ptyr-containing proteins. Those mutants that bound ptyr-containing proteins associated with tyrosine kinase activity. We also found that polypeptides containing SH2, SH2', and SH3 of p60v-src and p60c-src associated with ptyr-containing proteins from crk-transformed cells. Thus, the SH2 and SH2' domains of P47gag-crk are responsible for their binding to ptyr-containing proteins.

Related Genes
c-src v-crk v-src
MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic/genetics Cloning, Molecular DNA Mutational Analysis Oncogene Protein pp60(v-src)/metabolism Oncogene Protein v-crk Phosphoproteins/metabolism Phosphotyrosine Protein-Tyrosine Kinases/metabolism Proto-Oncogene Proteins pp60(c-src)/metabolism Rats Retroviridae Proteins, Oncogenic/genetics,metabolism Structure-Activity Relationship Tyrosine/analogs & derivatives,metabolism Viral Fusion Proteins/genetics,metabolism
Chemicals
Oncogene Protein v-crk Phosphoproteins Retroviridae Proteins, Oncogenic Viral Fusion Proteins Phosphotyrosine Tyrosine Protein-Tyrosine Kinases Oncogene Protein pp60(v-src) Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Matsuda M
Rockefeller University, New York, New York 10021.
Mayer B J
Hanafusa H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.3.1607-1613.1991
Published
1991-03-00
Pages
1607-13
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369454
Subset
IM
Grants
NIAID NIH HHS · AI07233 · United States
NCI NIH HHS · CA44356 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.3.1607-1613.1991 PMC Full Text View journal details
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