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PMID: 1705514 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Conserved V(D)J junctional sequence of cross-reactive cytotoxic T cell receptor idiotype and the effect of a single amino acid substitution.

European journal of immunology ·Vol. 21 ·No. 2 ·1991-02-00 ·Pages 483-8

Hirama T, Takeshita S, Matsubayashi Y, Iwashiro M, Masuda T, Kuribayashi K, Yoshida Y, Yamagishi H

Abstract

A dominant T cell population bearing the cross-reactive idiotype of T cell antigen receptor (TcR) has been obtained using an anti-TcR monoclonal antibody (mAb) developed in syngeneic mice. Forty-four cytotoxic T cell (CTL) clones with reactivity to a mAb (N9-127) were selected out of 396 H-2Db-restricted CTL clones specific for FBL-3 tumor antigen from C57BL/6 mice. These CTL clones were divided into two groups according to the blocking pattern of cytotoxic activities with mAb N9-127. All eight CTL clones chosen from both groups expressed TcR with a specific combination of alpha and beta chains (V alpha 1J alpha 112-2/V beta 10D beta 2.1J beta 2.7), and the difference in the blocking susceptibility resided in a single amino acid substitution (Gly to Asp) in the D-J joint of beta chain. This provides direct evidence for the molecular basis of cross-reactive idiotypes of TcR recognized by mAb.

MeSH Terms

Amino Acid Sequence Animals Antibodies, Monoclonal Base Sequence Clone Cells Cloning, Molecular Cross Reactions Cytotoxicity, Immunologic/immunology Epitopes/chemistry Mice Mice, Inbred C57BL Molecular Sequence Data Receptors, Antigen, T-Cell/chemistry,physiology Receptors, Antigen, T-Cell, alpha-beta Structure-Activity Relationship T-Lymphocytes, Cytotoxic/immunology

Chemicals

Antibodies, Monoclonal Epitopes Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, alpha-beta

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Hirama T

Department of Biophysics, Faculty of Science, Kyoto University, Japan.

Takeshita S

Matsubayashi Y

Iwashiro M

Masuda T

Kuribayashi K

Yoshida Y

Yamagishi H

Article Info

Journal

European journal of immunology

Abbr.

Eur J Immunol

ISSN

0014-2980

DOI

10.1002/eji.1830210234

Published

1991-02-00

Pages

483-8

Language

English

Region

Germany

NLM ID

1273201

Subset

Databases

GENBANK

X56701, X56702, X56703, X56704, X56705, X56706, X56707, X56708, X56709, X56710, X56711, X56712, X56713, X56714, X56715, X56716, X56717, X56718, X56719, X56720, X56721, X56722, X56723, X56724, X56725

External Links

PubMed source DOI: 10.1002/eji.1830210234 View journal details

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Conserved V(D)J junctional sequence of cross-reactive cytotoxic T cell receptor idiotype and the effect of a single amino acid substitution.

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