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PMID: 1705653 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mitochondrial genome of fission yeast: inability of all introns to splice autocatalytically, and construction and characterization of an intronless genome.

Molecular & general genetics : MGG ·Vol. 225 ·No. 1 ·1991-01-00 ·Pages 158-67

Schäfer B, Merlos-Lange AM, Anderl C, Welser F, Zimmer M, Wolf K

Abstract

In this paper we report the inability of four group I introns in the gene encoding subunit I of cytochrome c oxidase (cox1) and the group II intron in the apocytochrome b gene (cob) to splice autocatalytically. Furthermore we present the characterization of the first cox1 intron in the mutator strain anar-14 and the construction and characterization of strains with intronless mitochondrial genomes. We provide evidence that removal of introns at the DNA level (termed DNA splicing) is dependent on an active RNA maturase. Finally we demonstrate that the absence of introns does not abolish homologous mitochondrial recombination.

Related Genes
cob cox1
MeSH Terms
Amino Acid Sequence Apoproteins/genetics Base Sequence Cloning, Molecular Consensus Sequence Cytochrome b Group/genetics Cytochromes b DNA, Mitochondrial/genetics Electron Transport Complex IV/genetics Introns Mitochondria/metabolism Molecular Sequence Data Nucleic Acid Conformation RNA/genetics RNA Splicing RNA, Mitochondrial Schizosaccharomyces/genetics
Chemicals
Apoproteins Cytochrome b Group DNA, Mitochondrial RNA, Mitochondrial RNA Cytochromes b Electron Transport Complex IV
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schäfer B
Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität, Mainz, Federal Republic of Germany.
Merlos-Lange A M
Anderl C
Welser F
Zimmer M
Wolf K
References (30)
30 references, click to expand
  1. Self-splicing of yeast mitochondrial ribosomal and messenger RNA precursors.
    Cell. 1985 Apr;40(4):759-66 PMID: 2580635
  2. Distribution of mitochondrial introns in the species Schizosaccharomyces pombe and the origin of the group II intron in the gene encoding apocytochrome b.
    Curr Genet. 1987;12(5):329-36 PMID: 3446375
  3. Mitochondrial introns aI1 and/or aI2 are needed for the in vivo deletion of intervening sequences.
    Mol Gen Genet. 1989 May;217(1):168-71 PMID: 2475753
  4. Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure.
    Biochimie. 1982 Oct;64(10):867-81 PMID: 6817818
  5. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  6. The mitochondrial genome of the fission yeast Schizosaccharomyces pombe. The cytochrome b gene has an intron closely related to the first two introns in the Saccharomyces cerevisiae cox1 gene.
    J Mol Biol. 1985 Aug 5;184(3):353-66 PMID: 4046021
  7. Internal structure of a mitochondrial intron of Aspergillus nidulans.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6332-6 PMID: 6755468
  8. Self-splicing of group II introns in vitro: lariat formation and 3' splice site selection in mutant RNAs.
    Cell. 1987 Dec 4;51(5):753-62 PMID: 2445492
  9. Structural conventions for group I introns.
    Nucleic Acids Res. 1987 Sep 25;15(18):7217-21 PMID: 3658691
  10. The absence of introns in yeast mitochondria does not abolish mitochondrial recombination.
    Curr Genet. 1990 Jun;17(6):537-41 PMID: 2202528
  11. Group I introns as mobile genetic elements: facts and mechanistic speculations--a review.
    Gene. 1989 Oct 15;82(1):91-114 PMID: 2555264
  12. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  13. DNA sequence analysis of the 24.5 kilobase pair cytochrome oxidase subunit I mitochondrial gene from Podospora anserina: a gene with sixteen introns.
    Curr Genet. 1989 Dec;16(5-6):381-406 PMID: 2558809
  14. Extrachromosomal mutator inducing point mutations and deletions in mitochondrial genome of fission yeast.
    Proc Natl Acad Sci U S A. 1982 Apr;79(8):2618-22 PMID: 6953418
  15. Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron.
    Cell. 1980 Nov;22(2 Pt 2):333-48 PMID: 7004642
  16. The mitochondrial genome of the fission yeast Schizosaccharomyces pombe. 2. Localization of genes by interspecific hybridization in strain ade7-50h- and cloning of the genome in small fragments.
    Mol Gen Genet. 1984;196(3):465-72 PMID: 6094974
  17. Construction of a yeast strain devoid of mitochondrial introns and its use to screen nuclear genes involved in mitochondrial splicing.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6810-4 PMID: 3309947
  18. Two intron sequences in yeast mitochondrial COX1 gene: homology among URF-containing introns and strain-dependent variation in flanking exons.
    Cell. 1983 Feb;32(2):379-89 PMID: 6297792
  19. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  20. DNA sequence analysis of the apocytochrome b gene of Podospora anserina: a new family of intronic open reading frame.
    Curr Genet. 1989 Dec;16(5-6):407-18 PMID: 2611913
  21. Two changes of the same nucleotide confer resistance to diuron and antimycin in the mitochondrial cytochrome b gene of Schizosaccharomyces pombe.
    FEBS Lett. 1988 Sep 12;237(1-2):31-4 PMID: 3169239
  22. Mitochondrial class II introns encode proteins related to the reverse transcriptases of retroviruses.
    Nature. 1985 Aug 15-21;316(6029):641-3 PMID: 2412125
  23. The mosaic cox1 gene in the mitochondrial genome of Schizosaccharomyces pombe: minimal structural requirements and evolution of group I introns.
    Gene. 1986;45(3):289-97 PMID: 3026914
  24. The mitochondrial reading frame RF3 is a functional gene in Saccharomyces uvarum.
    J Biol Chem. 1987 Jul 25;262(21):10146-53 PMID: 2440860
  25. Analysis of class I introns in a mitochondrial plasmid associated with senescence of Podospora anserina reveals extraordinary resemblance to the Tetrahymena ribosomal intron.
    Curr Genet. 1985;10(1):69-79 PMID: 3940064
  26. Splicing of large ribosomal precursor RNA and processing of intron RNA in yeast mitochondria.
    Cell. 1984 Dec;39(3 Pt 2):623-9 PMID: 6210151
  27. The mitochondrial genome of the fission yeast Schizosaccharomyces pombe: highly homologous introns are inserted at the same position of the otherwise less conserved cox1 genes in Schizosaccharomyces pombe and Aspergillus nidulans.
    EMBO J. 1984 Sep;3(9):2129-36 PMID: 6092057
  28. Two distinct mechanisms for deletion in mitochondrial DNA of Schizosaccharomyces pombe mutator strains. Slipped mispairing mediated by direct repeats and erroneous intron splicing.
    J Mol Biol. 1988 Aug 20;202(4):725-34 PMID: 3172236
  29. The mitochondrial genome of the fission yeast Schizosaccharomyces pombe : 5. Characterization of mitochondrial deletion mutants.
    Curr Genet. 1984 Sep;8(7):517-24 PMID: 24177952
  30. Self-splicing of group II introns in vitro: mapping of the branch point and mutational inhibition of lariat formation.
    Cell. 1986 Aug 15;46(4):557-65 PMID: 3524857
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00282654
Published
1991-01-00
Pages
158-67
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
M64566, S63796, S63798, S79268, S79270, S79277, S85222, S85224, X57664, X57666
External Links
PubMed source DOI: 10.1007/BF00282654
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