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PMID: 1706732 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of coagulase-negative staphylococci by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and rRNA restriction patterns.

Journal of clinical microbiology ·Vol. 29 ·No. 2 ·1991-02-00 ·Pages 390-2

Pennington TH, Harker C, Thomson-Carter F

Abstract

A total of 1,417 staphylococcal and micrococcal strains were collected from the beards and scalps of 10 subjects over a period of 8 months. Sixteen strains identified as Staphylococcus epidermidis with an API system had distinctive yellow colonies on nutrient agar plates and sodium dodecyl sulfate-polyacrylamide gel electrophoresis whole-cell polypeptide profiles similar to those of Staphylococcus capitis; this identification was confirmed by analysis of rRNA gene restriction patterns.

MeSH Terms
Bacterial Proteins/isolation & purification Bacterial Typing Techniques Bacteriological Techniques Coagulase/analysis Electrophoresis, Polyacrylamide Gel Humans Polymorphism, Restriction Fragment Length RNA, Bacterial/genetics RNA, Ribosomal/genetics Staphylococcus/classification,genetics,isolation & purification
Chemicals
Bacterial Proteins Coagulase RNA, Bacterial RNA, Ribosomal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pennington T H
Department of Medical Microbiology, University of Aberdeen, Scotland.
Harker C
Thomson-Carter F
References (8)
8 references, click to expand
  1. Differentiation of staphylococcal species and strains by ribosomal RNA gene restriction patterns.
    J Gen Microbiol. 1989 Jul;135(7):2093-7 PMID: 2482338
  2. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools.
    Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165-75 PMID: 2446639
  3. Temporal study of staphylococcal species on the skin of human subjects in isolation and clonal analysis of Staphylococcus capitis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Clin Microbiol. 1989 Dec;27(12):2627-32 PMID: 2687313
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. Staphylococcal whole-cell polypeptide analysis: evaluation as a taxonomic and typing tool.
    J Med Microbiol. 1987 Feb;23(1):41-4 PMID: 3820270
  6. Identification of coagulase-negative staphylococci with the API staph system.
    J Clin Microbiol. 1982 Nov;16(5):874-7 PMID: 6924937
  7. Simplified scheme for routine identification of human Staphylococcus species.
    J Clin Microbiol. 1975 Jan;1(1):82-8 PMID: 170303
  8. Evaluation of a conventional routine method for identification of clinical isolates of coagulase-negative Staphylococcus and Micrococcus species. Comparison with API-Staph and API-Staph-Ident.
    Acta Pathol Microbiol Immunol Scand B. 1987 Oct;95(5):283-92 PMID: 3314337
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.29.2.390-392.1991
Published
1991-02-00
Pages
390-2
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC269773
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.29.2.390-392.1991 PMC Full Text View journal details
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