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PMID: 1707539 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Acidic and basic fibroblast growth factors are survival factors with distinctive activity in quiescent BALB/c 3T3 murine fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 8 ·1991-04-15 ·Pages 3372-6

Tamm I, Kikuchi T, Zychlinsky A

Abstract

Platelet-derived growth factor (PDGF), epidermal growth factor, and insulin-like growth factor have previously been identified as survival factors with distinctive activities for the density-inhibited quiescent BALB/c 3T3 murine fibroblasts. Fibroblast growth factor (FGF), like PDGF, renders quiescent BALB/c 3T3 cells competent to respond to epidermal growth factor and insulin-like growth factor, which mediate cell-cycle traverse through G1 into S phase [Stiles, C. D., Pledger, W. J., VanWyk, J. J., Antoniades, H. N. & Scher, C. D. (1979) Proc. Natl. Acad. Sci. USA 76, 1279-1283]. We now show that FGF possess marked cell survival-enhancing activity distinctive from that of PDGF. Both acidic FGF (aFGF) and basic FGF (bFGF) markedly enhance short-term (3-hr) survival of quiescent cells. bFGF is the more active of the two factors and shows marked long-term (20-hr) survival-promoting activity alone, whereas aFGF requires heparin for long-term activity. Protection by bFGF or aFGF plus heparin is not associated with cell-cycle traverse into S phase. Both the short-term (3-hr) and long-term (20-hr) protective actions of aFGF and bFGF critically depend on protein synthesis, whereas those of PDGF do not. The accumulated evidence shows that several growth factors can contribute to maintenance of the integrity of quiescent murine fibroblasts and that their action can involve protein kinase A- and C-mediated processes as well as protein synthesis. Different growth factors display distinctive modes of action.

MeSH Terms
Alkaloids/pharmacology Animals Cell Line Cell Survival/drug effects Culture Media Cycloheximide/pharmacology DNA Damage Fibroblast Growth Factor 1/pharmacology Fibroblast Growth Factor 2/pharmacology Heparin/pharmacology In Vitro Techniques Mice Nucleosomes/ultrastructure Platelet-Derived Growth Factor/pharmacology RNA/biosynthesis Staurosporine
Chemicals
Alkaloids Culture Media Nucleosomes Platelet-Derived Growth Factor Fibroblast Growth Factor 2 Fibroblast Growth Factor 1 RNA Heparin Cycloheximide Staurosporine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tamm I
Laboratories of Cell Physiology, Rockefeller University, New York, NY 10021.
Kikuchi T
Zychlinsky A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.8.3372
Published
1991-04-15
Pages
3372-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51449
Subset
IM
Grants
NCI NIH HHS · CA-18608 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.8.3372 PMC Full Text View journal details
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