Polyamine induced aggregation of DNA.

Nucleic acids research ·Vol. 4 ·No. 3 ·1977-03-00 ·Pages 685-95

Osland A, Kleppe K

Abstract

Polyamine induced aggregation of various DNAs has been studied under conditions usually employed in many enzymatic assays where DNA is one of the substrates. Spermine was by far the most efficient polyamine in causing aggregation followed by spermidine and cadaverine. All double-stranded and naturally occurring single-stranded DNAs were found to aggregate. No aggregation of single-stranded homodeoxypolymers could be detected under the same conditions. The concentration of polyamine at which the aggregation commenced was found to be a linear function of the DNA concentration. The slope of the curves depended on the nature of the polyamine, DNA the concentration of Mg++ and the ionic strength.

MeSH Terms

Cadaverine/pharmacology Coliphages DNA/metabolism DNA, Single-Stranded/metabolism DNA, Viral/metabolism Hydrogen-Ion Concentration Magnesium/pharmacology Molecular Weight Polyamines/pharmacology Polydeoxyribonucleotides/metabolism Potassium Chloride/pharmacology Species Specificity Spermidine/pharmacology Spermine/pharmacology Structure-Activity Relationship

Chemicals

DNA, Single-Stranded DNA, Viral Polyamines Polydeoxyribonucleotides Spermine Potassium Chloride DNA Magnesium Cadaverine Spermidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Osland A

Kleppe K

References (8)

8 references, click to expand

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The reaction of formaldehyde with nucleotides and T2 bacteriophage DNA.
J Mol Biol. 1961 Feb;3:47-60 PMID: 13709093
Solubility of complexes of polynucleotides with spermine.
Nature. 1960 Oct 22;188:301-2 PMID: 13716401
Effect of salts and polyamines on T4 polynucleotide kinase.
Biochemistry. 1975 Mar 25;14(6):1225-9 PMID: 164211
Kinetics and effect of salts and polyamines on T4 polynucleotide ligase.
Eur J Biochem. 1975 Dec 15;60(2):437-43 PMID: 173544
Observations on the affinity between polyamines and nucleic acids.
Hoppe Seylers Z Physiol Chem. 1971 Jan;352(1):29-38 PMID: 5099699
Interaction between spermine and histones at nucleoprotein formation.
Hoppe Seylers Z Physiol Chem. 1971 Jan;352(1):39-42 PMID: 5099700
Reactions of quinine, chloroquine, and quinacrine with DNA and their effects on the DNA and RNA polymerase reactions.
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1511-7 PMID: 5336287

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.3.685

Published

1977-03-00

Pages

685-95

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342472

Subset

External Links

PubMed source DOI: 10.1093/nar/4.3.685 PMC Full Text View journal details

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Polyamine induced aggregation of DNA.

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