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PMID: 17105821 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Gene expression patterns in blood leukocytes discriminate patients with acute infections.

Blood ·Vol. 109 ·No. 5 ·2007-03-01 ·Pages 2066-77

Ramilo O, Allman W, Chung W, Mejias A, Ardura M, Glaser C, Wittkowski KM, Piqueras B, Banchereau J, Palucka AK, Chaussabel D

Abstract

Each infectious agent represents a unique combination of pathogen-associated molecular patterns that interact with specific pattern-recognition receptors expressed on immune cells. Therefore, we surmised that the blood immune cells of individuals with different infections might bear discriminative transcriptional signatures. Gene expression profiles were obtained for 131 peripheral blood samples from pediatric patients with acute infections caused by influenza A virus, Gram-negative (Escherichia coli) or Gram-positive (Staphylococcus aureus and Streptococcus pneumoniae) bacteria. Thirty-five genes were identified that best discriminate patients with influenza A virus infection from patients with either E coli or S pneumoniae infection. These genes classified with 95% accuracy (35 of 37 samples) an independent set of patients with either influenza A, E coli, or S pneumoniae infection. A different signature discriminated patients with E coli versus S aureus infections with 85% accuracy (34 of 40). Furthermore, distinctive gene expression patterns were observed in patients presenting with respiratory infections of different etiologies. Thus, microarray analyses of patient peripheral blood leukocytes might assist in the differential diagnosis of infectious diseases.

MeSH Terms
Acute Disease Adolescent Cell Separation Child Child, Preschool Communicable Diseases/blood,diagnosis,genetics Escherichia coli Infections/classification,genetics,microbiology Female Gene Expression Profiling Gene Expression Regulation Health Humans Infant Infant, Newborn Leukocytes/metabolism Male Oligonucleotide Array Sequence Analysis Respiratory Tract Infections/classification,genetics,microbiology Staphylococcal Infections/classification,genetics,microbiology Transcription, Genetic/genetics
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Ramilo Octavio
Baylor National Institute of Allergy and Infections Diseases (NIAID) Cooperative Center for Translational Research on Human Immunology and Biodefense and Baylor Institute for Immunology Research, Dallas, TX 75204, USA. [email protected]
Allman Windy
Chung Wendy
Mejias Asuncion
Ardura Monica
Glaser Casey
Wittkowski Knut M
Piqueras Bernard
Banchereau Jacques
Palucka A Karolina
Chaussabel Damien
References (43)
43 references, click to expand
  1. Stereotyped and specific gene expression programs in human innate immune responses to bacteria.
    Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):972-7 PMID: 11805339
  2. Suppression of the interferon-mediated innate immune response by pseudorabies virus.
    J Virol. 2006 Jul;80(13):6345-56 PMID: 16775323
  3. Molecular regulation of urothelial renewal and host defenses during infection with uropathogenic Escherichia coli.
    J Biol Chem. 2002 Mar 1;277(9):7412-9 PMID: 11744708
  4. New technologies, human-microbe interactions, and the search for previously unrecognized pathogens.
    J Infect Dis. 2002 Dec 1;186 Suppl 2:S254-8 PMID: 12424706
  5. A gene-expression signature as a predictor of survival in breast cancer.
    N Engl J Med. 2002 Dec 19;347(25):1999-2009 PMID: 12490681
  6. Calcium-binding proteins MRP 8 and 14 in a Staphylococcus aureus infection model: role of therapy, inflammation, and infection persistence.
    J Lab Clin Med. 2003 Feb;141(2):110-20 PMID: 12577047
  7. Interferon-inducible gene expression signature in peripheral blood cells of patients with severe lupus.
    Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2610-5 PMID: 12604793
  8. Interferon and granulopoiesis signatures in systemic lupus erythematosus blood.
    J Exp Med. 2003 Mar 17;197(6):711-23 PMID: 12642603
  9. Unique gene expression profiles of human macrophages and dendritic cells to phylogenetically distinct parasites.
    Blood. 2003 Jul 15;102(2):672-81 PMID: 12663451
  10. Molecular signatures for diagnosis of infection: application of microarray technology.
    J Appl Microbiol. 2004;96(1):18-23 PMID: 14678155
  11. Chips with everything: DNA microarrays in infectious diseases.
    Lancet Infect Dis. 2004 Feb;4(2):100-11 PMID: 14871635
  12. LPS, dsRNA and the interferon bridge to adaptive immune responses: Trif, Tram, and other TIR adaptor proteins.
    J Endotoxin Res. 2004;10(2):130-6 PMID: 15120005
  13. Localization of serglycin in human neutrophil granulocytes and their precursors.
    J Leukoc Biol. 2004 Aug;76(2):406-15 PMID: 15136585
  14. Transcriptional activation induced in macrophages by Toll-like receptor (TLR) ligands: from expression profiling to a model of TLR signaling.
    Eur J Immunol. 2004 Oct;34(10):2863-73 PMID: 15368303
  15. Emerging infectious diseases: a clear and present danger to humanity.
    JAMA. 2004 Oct 20;292(15):1887-8 PMID: 15494589
  16. The host response to smallpox: analysis of the gene expression program in peripheral blood cells in a nonhuman primate model.
    Proc Natl Acad Sci U S A. 2004 Oct 19;101(42):15190-5 PMID: 15477590
  17. Prolonged toll-like receptor signaling by Mycobacterium tuberculosis and its 19-kilodalton lipoprotein inhibits gamma interferon-induced regulation of selected genes in macrophages.
    Infect Immun. 2004 Nov;72(11):6603-14 PMID: 15501793
  18. The interleukin-8-related chemotactic cytokines GRO alpha, GRO beta, and GRO gamma activate human neutrophil and basophil leukocytes.
    J Biol Chem. 1993 Jul 25;268(21):15419-24 PMID: 8340371
  19. Granulocyte/macrophage colony-stimulating factor stimulates the expression of the 5-lipoxygenase-activating protein (FLAP) in human neutrophils.
    J Exp Med. 1994 Apr 1;179(4):1225-32 PMID: 8145039
  20. Innate immunity: the virtues of a nonclonal system of recognition.
    Cell. 1997 Oct 31;91(3):295-8 PMID: 9363937
  21. Molecular classification of cancer: class discovery and class prediction by gene expression monitoring.
    Science. 1999 Oct 15;286(5439):531-7 PMID: 10521349
  22. Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling.
    Nature. 2000 Feb 3;403(6769):503-11 PMID: 10676951
  23. Innate immune recognition: mechanisms and pathways.
    Immunol Rev. 2000 Feb;173:89-97 PMID: 10719670
  24. Increased expression of matrix metalloproteinase-9 in neutrophils in glycogen-induced peritoneal inflammation of guinea pigs.
    Inflamm Res. 2000 Feb;49(2):55-62 PMID: 10738943
  25. Herpes simplex virus triggers and then disarms a host antiviral response.
    J Virol. 2001 Jan;75(2):750-8 PMID: 11134288
  26. The plasticity of dendritic cell responses to pathogens and their components.
    Science. 2001 Oct 26;294(5543):870-5 PMID: 11679675
  27. CD147 is a signaling receptor for cyclophilin B.
    Biochem Biophys Res Commun. 2001 Nov 9;288(4):786-8 PMID: 11688976
  28. Insights into host responses against pathogens from transcriptional profiling.
    Nat Rev Microbiol. 2005 Apr;3(4):281-94 PMID: 15806094
  29. Effect of heptitis C virus core protein on cellular gene expression: specific inhibition of cyclooxygenase 2.
    J Infect Dis. 2005 May 1;191(9):1498-506 PMID: 15809909
  30. Genomewide analysis of the host response to malaria in Kenyan children.
    J Infect Dis. 2005 May 15;191(10):1599-611 PMID: 15838786
  31. Independence and reproducibility across microarray platforms.
    Nat Methods. 2005 May;2(5):337-44 PMID: 15846360
  32. Multiple-laboratory comparison of microarray platforms.
    Nat Methods. 2005 May;2(5):345-50 PMID: 15846361
  33. Standardizing global gene expression analysis between laboratories and across platforms.
    Nat Methods. 2005 May;2(5):351-6 PMID: 15846362
  34. Functional genomic relationships in HIV-1 disease revealed by gene-expression profiling of primary human peripheral blood mononuclear cells.
    J Infect Dis. 2005 Jun 15;191(12):2064-74 PMID: 15897992
  35. Common and unique gene expression signatures of human macrophages in response to four strains of Mycobacterium avium that differ in their growth and persistence characteristics.
    Infect Immun. 2005 Jun;73(6):3330-41 PMID: 15908359
  36. Expression of genes encoding innate host defense molecules in normal human monocytes in response to Candida albicans.
    Infect Immun. 2005 Jun;73(6):3714-24 PMID: 15908401
  37. The global challenge of infectious diseases: the evolving role of the National Institutes of Health in basic and clinical research.
    Nat Immunol. 2005 Aug;6(8):743-7 PMID: 16034426
  38. Surveillance of transcriptomes in basic military trainees with normal, febrile respiratory illness, and convalescent phenotypes.
    Genes Immun. 2005 Oct;6(7):588-95 PMID: 16034474
  39. Functional genomics of innate host defense molecules in normal human monocytes in response to Aspergillus fumigatus.
    Infect Immun. 2006 Apr;74(4):2353-65 PMID: 16552065
  40. Upon viral exposure, myeloid and plasmacytoid dendritic cells produce 3 waves of distinct chemokines to recruit immune effectors.
    Blood. 2006 Apr 1;107(7):2613-8 PMID: 16317096
  41. Human rhinovirus attenuates the type I interferon response by disrupting activation of interferon regulatory factor 3.
    J Virol. 2006 May;80(10):5021-31 PMID: 16641293
  42. Inhibition of the NF-kappaB pathway by varicella-zoster virus in vitro and in human epidermal cells in vivo.
    J Virol. 2006 Jun;80(11):5113-24 PMID: 16698992
  43. Human macrophage activation programs induced by bacterial pathogens.
    Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1503-8 PMID: 11805289
Article Info
Journal
Blood
Abbr.
Blood
ISSN
0006-4971
DOI
10.1182/blood-2006-02-002477
Email
[email protected]
Published
2007-03-01
Epub
2006-00-14
Pages
2066-77
Language
English
Region
United States
NLM ID
7603509
PMCID
PMC1801073
Subset
IM
Grants
NCI NIH HHS · R01 CA078846 · United States
NIAID NIH HHS · U19 AI057234 · United States
NCI NIH HHS · CA78846 · United States
NIAID NIH HHS · U19 AI057234-02 · United States
External Links
PubMed source DOI: 10.1182/blood-2006-02-002477 PMC Full Text View journal details
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