Home LiteratureArticle Details
PMID: 1710619 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Further characterizations of bleomycin-sensitive (blm) mutants of Saccharomyces cerevisiae with implications for a radiomimetic model.

Journal of bacteriology ·Vol. 173 ·No. 11 ·1991-06-00 ·Pages 3605-8

Moore CW

Abstract

Direct selection for 12 mutations (blm) conferring hypersensitivities to lethal effects of bleomycins in Saccharomyces cerevisiae resulted in mutants exhibiting cross-hypersensitivity to ionizing radiation and hydrogen peroxide. Remaining mutations did not confer cross-hypersensitivity to radiation. All blm mutations were recessive, except codominant blm3-1, and were assigned to seven complementation groups.

Related Genes
blm
MeSH Terms
Bleomycin/pharmacology Cell Survival/drug effects Chromosome Mapping DNA/drug effects,radiation effects Dose-Response Relationship, Drug Drug Resistance, Microbial/genetics Genetic Complementation Test Hydrogen Peroxide/pharmacology Radiation Tolerance Radiation, Ionizing Saccharomyces cerevisiae/genetics Ultraviolet Rays/adverse effects
Chemicals
Bleomycin DNA Hydrogen Peroxide
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Moore C W
City University of New York, Department of Microbiology, Medical School, New York 10031.
References (25)
25 references, click to expand
  1. Interactions of bleomycin with DNA.
    Adv Enzyme Regul. 1980;18:67-83 PMID: 6160737
  2. Role of DNA sequences in genetic recombination in the iso-1-cytochrome c gene of yeast. I. Discrepancies between physical distances and genetic distances determined by five mapping procedures.
    Genetics. 1975 Mar;79(3):397-418 PMID: 165136
  3. Analysis of products formed during bleomycin-mediated DNA degradation.
    Biochemistry. 1985 Oct 8;24(21):5735-44 PMID: 2417619
  4. Bleomycin-induced DNA repair synthesis in permeable human fibroblasts: mediation of long-patch and short-patch repair by distinct DNA polymerases.
    Biochemistry. 1989 Nov 28;28(24):9515-20 PMID: 2482074
  5. Yeast DNA 3'-repair diesterase is the major cellular apurinic/apyrimidinic endonuclease: substrate specificity and kinetics.
    J Biol Chem. 1988 Dec 5;263(34):18017-22 PMID: 3056936
  6. Yeast DNA diesterase for 3'-fragments of deoxyribose: purification and physical properties of a repair enzyme for oxidative DNA damage.
    J Biol Chem. 1988 Dec 5;263(34):18009-16 PMID: 3056935
  7. Enhanced bleomycin-mediated damage of DNA opposite charged nicks. A model for bleomycin-directed double strand scission of DNA.
    J Biol Chem. 1987 Nov 5;262(31):15144-50 PMID: 2444595
  8. Exonuclease III and endonuclease IV remove 3' blocks from DNA synthesis primers in H2O2-damaged Escherichia coli.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7731-5 PMID: 2429316
  9. Aphidicolin-sensitive DNA repair synthesis in human fibroblasts damaged with bleomycin is distinct from UV-induced repair.
    Carcinogenesis. 1990 Jun;11(6):1021-6 PMID: 1693320
  10. Bleomycin-induced strand-scission of DNA. Mechanism of deoxyribose cleavage.
    J Biol Chem. 1981 Aug 25;256(16):8608-15 PMID: 6167580
  11. Ligase-deficient yeast cells exhibit defective DNA rejoining and enhanced gamma ray sensitivity.
    J Bacteriol. 1982 Jun;150(3):1227-33 PMID: 7042687
  12. cdc9 ligase-defective mutants of Saccharomyces cerevisiae exhibit lowered resistance to lethal effects of bleomycin.
    J Bacteriol. 1982 Sep;151(3):1617-20 PMID: 6179928
  13. Enzyme action at 3' termini of ionizing radiation-induced DNA strand breaks.
    J Biol Chem. 1983 Dec 25;258(24):15198-205 PMID: 6361028
  14. Activated bleomycin. A transient complex of drug, iron, and oxygen that degrades DNA.
    J Biol Chem. 1981 Nov 25;256(22):11636-44 PMID: 6170635
  15. Isolation and partial characterization of mutants of Saccharomyces cerevisiae altered in sensitivities to lethal effects of bleomycins.
    J Antibiot (Tokyo). 1980 Nov;33(11):1369-75 PMID: 6166602
  16. Control of in vivo (cellular) phleomycin sensitivity by nuclear genotype, growth phase, and metal ions.
    Cancer Res. 1982 Mar;42(3):929-33 PMID: 6174219
  17. gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini.
    J Biol Chem. 1983 Jan 25;258(2):711-3 PMID: 6822504
  18. DNA-ferrous iron catalyzed hydroxyl free radical formation from hydrogen peroxide.
    Biochem Biophys Res Commun. 1981 Apr 30;99(4):1209-15 PMID: 7259775
  19. The damaging action of hydrogen peroxide on DNA of human fibroblasts is mediated by a non-dialyzable compound.
    Biochim Biophys Acta. 1980 Jun 27;608(1):167-73 PMID: 7388029
  20. Interaction of bleomycin with DNA.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5983-7 PMID: 83650
  21. Sequence specific cleavage of DNA by the antitumor antibiotics neocarzinostatin and bleomycin.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3608-12 PMID: 80799
  22. Responses of radiation-sensitive mutants of Saccharomyces cerevisiae to lethal effects of bleomycin.
    Mutat Res. 1978 Aug;51(2):165-80 PMID: 80746
  23. Genetic effects of impure and pure saccharin in yeast.
    Science. 1979 Sep 7;205(4410):1007-10 PMID: 382356
  24. Two forms of repair in the DNA of human cells damaged by chemical carcinogens and mutagens.
    Cancer Res. 1974 Dec;34(12):3318-25 PMID: 4371955
  25. Abnormal response of ataxia-telangiectasia cells to agents that break the deoxyribose moiety of DNA via a targeted free radical mechanism.
    Carcinogenesis. 1983 Oct;4(10):1317-22 PMID: 6616760
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.11.3605-3608.1991
Published
1991-06-00
Pages
3605-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207981
Subset
IM
Grants
NCI NIH HHS · CA25609 · United States
External Links
PubMed source DOI: 10.1128/jb.173.11.3605-3608.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2