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PMID: 1711221 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Developmental expression of the 25-kDa synaptosomal-associated protein (SNAP-25) in rat brain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 12 ·1991-06-15 ·Pages 5247-51

Oyler GA, Polli JW, Wilson MC, Billingsley ML

Abstract

The developmental expression and subcellular distribution of the neuron-specific 25-kDa synaptosomal protein (SNAP-25) were investigated by using Northern (RNA) blots, immunoblots, and immunocytochemistry. Both SNAP-25 protein and mRNA were present at low levels in embryonic day 15 rat brain, and levels of both increased during early postnatal maturation. Developmental immunoblots with antipeptide antisera demonstrated that a 25-kDa peptide was the major isoform in brain, and this form increased steadily from embryonic day 15 through adulthood. A second 27-kDa immunoreactive isoform was present in brain only during early development. Immunoblots of two-dimensional SDS/polyacrylamide gels revealed the presence of a predominant 25-kDa isoform of SNAP-25 in adult brain. Immunocytochemical studies indicated that as immunoreactivity for SNAP-25 increased during development, the cellular localization of SNAP-25 immunoreactivity concomitantly shifted from axons and cell bodies to presynaptic terminals. These data suggest that the SNAP-25 protein shifts in subcellular localization during development and may play a role in the establishment and stabilization of specific presynaptic terminals in brain.

MeSH Terms
Animals Blotting, Northern Brain/growth & development,metabolism Electrophoresis, Gel, Two-Dimensional Female Immunohistochemistry Membrane Proteins Nerve Tissue Proteins/metabolism RNA/analysis Rats Rats, Inbred Strains Synaptosomal-Associated Protein 25
Chemicals
Membrane Proteins Nerve Tissue Proteins Snap25 protein, rat Synaptosomal-Associated Protein 25 RNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Oyler G A
Department of Pharmacology Pennsylvania State University College of Medicine, Hershey 17033.
Polli J W
Wilson M C
Billingsley M L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.12.5247
Published
1991-06-15
Pages
5247-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51849
Subset
IM
Grants
NIA NIH HHS · R01-AG06377 · United States
NIEHS NIH HHS · R01-ES05450-01 · United States
NINDS NIH HHS · R01-NS23038 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.12.5247 PMC Full Text View journal details
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