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PMID: 1713248 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression and function of a variant T cell receptor complex lacking CD3-gamma.

The Journal of experimental medicine ·Vol. 174 ·No. 2 ·1991-08-01 ·Pages 319-26

Pérez-Aciego P, Alarcón B, Arnaiz-Villena A, Terhorst C, Timón M, Segurado OG, Regueiro JR

Abstract

A T cell line termed DIL2 has been derived from an infant with a polyclonal T cell receptor (TCR)/CD3 cell surface expression defect. Indirect immunofluorescence showed that the expression of certain TCR/CD3 epitopes (like those detected by WT31 and BMA031 monoclonals) was strongly reduced (around five-fold) on DIL2, whereas other epitopes (like those detected by SP34 and Leu4) were only around two-fold lower than in normal T cell lines. Specific immunoprecipitates of surface-radioiodinated DIL2 cells contained TCR-alpha, TCR-beta, CD3-delta, CD3-epsilon and TCR-zeta chains, but lacked CD3-gamma. This structural TCR/CD3 variant was, however, capable of transducing certain activation signals, since normal proliferation and a low but significant calcium flux was observed in DIL2 cells after engagement with specific antibodies. Our data suggest that a functional TCR/CD3 complex can be expressed on the surface of T cells in the absence of CD3-gamma.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, Differentiation, T-Lymphocyte/deficiency,immunology Blotting, Northern CD3 Complex Calcium/metabolism Cell Line Epitopes/immunology Flow Cytometry Gene Expression Humans Immunophenotyping Interleukin-2/biosynthesis Lymphocyte Activation/immunology RNA/analysis RNA, Messenger/metabolism Radioimmunoassay Receptors, Antigen, T-Cell/deficiency,immunology Receptors, Antigen, T-Cell, gamma-delta Signal Transduction T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte CD3 Complex Epitopes Interleukin-2 RNA, Messenger Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, gamma-delta RNA Calcium
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pérez-Aciego P
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
Alarcón B
Arnaiz-Villena A
Terhorst C
Timón M
Segurado O G
Regueiro J R
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.174.2.319
Published
1991-08-01
Pages
319-26
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2118903
Subset
IM
Grants
PHS HHS · IA-15066 · United States
External Links
PubMed source DOI: 10.1084/jem.174.2.319 PMC Full Text View journal details
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