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PMID: 1714935 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7.

The Journal of experimental medicine ·Vol. 174 ·No. 3 ·1991-09-01 ·Pages 625-31

Freeman GJ, Gray GS, Gimmi CD, Lombard DB, Zhou LJ, White M, Fingeroth JD, Gribben JG, Nadler LM

Abstract

Following occupancy of the T cell receptor by antigen, T cell proliferation and lymphokine production are determined by a second costimulatory signal delivered by a ligand expressed on antigen presenting cells. The human B cell activation antigen B7, which is expressed on antigen presenting cells including activated B cells and gamma interferon treated monocytes, has been shown to deliver such a costimulatory signal upon attachment to its ligand on T cells, CD28. We have cloned and sequenced the murine homologue of the human B7 gene. The predicted murine protein has 44% amino acid identity with human B7. The greatest similarity is in the Ig-V and Ig-C like domains. Murine B7 mRNA was detected in murine hematopoietic cells of B cell but not T cell origin. Cells transfected with murine B7 provided a costimulatory signal to human CD28+ T lymphocytes. These results demonstrate the costimulatory activity of murine B7 and provide evidence that the ligand attachment site is conserved between the two species.

MeSH Terms
Amino Acid Sequence Antigens, Differentiation, T-Lymphocyte/immunology Antigens, Surface/genetics,immunology B-Lymphocytes/immunology,physiology B7-1 Antigen Blotting, Northern Blotting, Southern CD28 Antigens Cloning, Molecular DNA/genetics Gene Expression Genes Humans Lymphocyte Activation Molecular Sequence Data Oligonucleotides/chemistry Polymerase Chain Reaction RNA, Messenger/genetics Receptors, Immunologic/immunology T-Lymphocyte Subsets/immunology
Chemicals
Antigens, Differentiation, T-Lymphocyte Antigens, Surface B7-1 Antigen CD28 Antigens Oligonucleotides RNA, Messenger Receptors, Immunologic DNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Freeman G J
Division of Tumor Immunology, Dana Farber Cancer Institute, Boston, Massachusetts.
Gray G S
Gimmi C D
Lombard D B
Zhou L J
White M
Fingeroth J D
Gribben J G
Nadler L M
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25 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.174.3.625
Published
1991-09-01
Pages
625-31
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2118924
Subset
IM
Grants
FIC NIH HHS · 1FO5 TWO4469 · United States
NCI NIH HHS · CA-40216 · United States
NIAID NIH HHS · R29AI-26835 · United States
Databases
GENBANK
S58757, S58759, S58761, S58763, S58765, S58776, S58778, S58779, S58781, X60958
External Links
PubMed source DOI: 10.1084/jem.174.3.625 PMC Full Text View journal details
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