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PMID: 171624 Published · ppublish English Journal Article

The role of extracellular Ca2+ and cyclic nucleotides in the mechanism of enzyme secretion from the cat pancreas.

Pflugers Archiv : European journal of physiology ·Vol. 360 ·No. 2 ·1975-10-28 ·Pages 165-81

Schulz I

Abstract

The role of Ca2+ in protein secretion from the isolated perfused cat's pancreas, the effect of the dibutyryl analogues of cAMP and cGMP, and the interrelation of Ca2+ and the nucleotides were studied. The following results were obtained: 1. Pancreatic enzyme secretion can be elicited by CaCl2 injections into the pancreatic arteries and is linearily related to the peak Ca2+ concentration in the effluent perfusate. Different background Ca2+ concentrations in the perfusate (3 mM or 0.125 mM) do not disturb this relation, indicating that no adaptation occurs. The effect of Ca2+ injections is of the same magnitude as that evoked by the hormones pancreozymin or acetylcholine. 2. Injections of Ca2+ potentiate the effects of dibutyryl adenosine 3',5'-cyclic monophosphate (dbcAMP), dibutyryl guanosine 3',5'-cyclic monophosphate (dbcGMP) or theophylline. 3. Infusion of low doses of pancreozymin increases the Ca2+ effect. The findings indicate that extracellular Ca2+ is involved in the mechanism of enzyme secretion and that Ca2+ and cyclic nucleotides have a synergistic action on the target.

MeSH Terms
Animals Calcium/pharmacology Cats Cyclic AMP/analogs & derivatives,pharmacology Cyclic GMP/analogs & derivatives,pharmacology Drug Synergism Enzyme Induction/drug effects Female Male Nucleotides, Cyclic/pharmacology Pancreas/drug effects,enzymology,metabolism Proteins/metabolism Theophylline/pharmacology
Chemicals
Nucleotides, Cyclic Proteins Theophylline Cyclic AMP Cyclic GMP Calcium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Schulz I
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34 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00580539
Published
1975-10-28
Pages
165-81
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00580539 View journal details
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