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PMID: 17165236 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The agarase gene (dagA) of Streptomyces coelicolor A3(2): nucleotide sequence and transcriptional analysis.

Molecular & general genetics : MGG ·Vol. 209 ·No. 1 ·1987-08-00 ·Pages 101-9

Buttner MJ, Fearnley IM, Bibb MJ

Abstract

The DNA sequence of a 1.77 kb region of the Streptomyces coelicolor chromosome containing the coding and regulatory regions of the extracellular agarase (dagA) gene was determined. The sequence predicts a primary translation product of 309 amino acids and Mr 35132. Comparison of the N-terminal sequence determined for the mature extracellular protein with that of the primary translation product deduced from the DNA sequence predicts the presence of a 30 amino acid signal peptide. Analysis of the transcription of the dagA gene using high resolution S1 mapping, in vitro transcription, dinucleotide-primed in vitro transcription and in vivo promoter probing identified four promoters, initiating transcription approximately 32, 77, 125 and 200 nucleotides upstream of the coding sequence.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Bacterial/genetics Genes, Bacterial/genetics Glycoside Hydrolases/genetics Promoter Regions, Genetic/genetics Protein Sorting Signals/genetics Streptomyces coelicolor/enzymology,genetics
Chemicals
DNA, Bacterial Protein Sorting Signals Glycoside Hydrolases agarase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Buttner M J
John Innes Institute, Colney Lane, Norwich NR4 7UH, UK.
Fearnley I M
Bibb M J
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00329843
Published
1987-08-00
Pages
101-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00329843
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