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PMID: 171657 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Enhanced representation of HL-A antigens on human lymphocytes after mitogenesis induced by phytohemagglutinin or Epstein-Barr virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 8 ·1975-08-00 ·Pages 3206-9

McCune JM, Humphreys RE, Yocum RR, Strominger JL

Abstract

The amount of HL-A antigens present on the peripheral blood lymphocytes of a single human donor was increased about 11-fold after stimulation with phytohemagglutinin and 36-fold after transformation with Epstein-Barr virus. This increase applied to all four HL-A specificities of these cells. The response to phytohemagglutinin was dependent on dose and was first observed at 12 hr of incubation. Measurements of the amount of surface membranes by geometry, by radioiodinatable surface proteins, and by 5'-nucleotidase (5'-ribonucleotide phosphohydrolase, EC 3.1.3.5) assay all indicated that the enhanced representation of HL-A antigens after stimulation by phytohemagglutinin or transformation by Epstein-Barr virus must be due to an increase in the density of these antigens on the cell surface.

MeSH Terms
Binding Sites Cell Membrane/metabolism,ultrastructure Cell Transformation, Neoplastic HLA Antigens/metabolism Herpesvirus 4, Human/physiology Histocompatibility Antigens/metabolism Humans Lectins/pharmacology Lymphocytes/metabolism
Chemicals
HLA Antigens Histocompatibility Antigens Lectins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McCune J M
Humphreys R E
Yocum R R
Strominger J L
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.8.3206
Published
1975-08-00
Pages
3206-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432951
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.8.3206 PMC Full Text View journal details
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