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PMID: 1717018 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Time course of receptor-channel coupling in frog sympathetic neurons.

Biophysical journal ·Vol. 60 ·No. 2 ·1991-08-00 ·Pages 502-7

Jones SW

Abstract

The M-type potassium current and the N-type calcium current are inhibited by several different neurotransmitters in frog sympathetic neurons. These effects seem to be mediated via G proteins, but it is not clear whether diffusible second messengers are involved. Using a rapid (approximately 100 ms) flow tube perfusion system to apply agonists, the inhibition of calcium current develops and recovers rapidly but not instantaneously (t1/2 = 1-2 s). M-current inhibition is considerably slower, with t1/2 approximately 30 s for recovery from inhibition. At least for M-current inhibition, there appears to be sufficient time for involvement of an enzymatic cascade in receptor-channel coupling.

MeSH Terms
Animals Biophysical Phenomena Biophysics Calcium Channels/drug effects,metabolism Electrochemistry Gonadotropin-Releasing Hormone/pharmacology In Vitro Techniques Ion Channels/drug effects,metabolism Kinetics Neurons/metabolism Neurotransmitter Agents/pharmacology Potassium Channels/drug effects,metabolism Rana catesbeiana Receptors, Neurotransmitter/drug effects,metabolism Second Messenger Systems/drug effects,physiology Sympathetic Nervous System/metabolism
Chemicals
Calcium Channels Ion Channels Neurotransmitter Agents Potassium Channels Receptors, Neurotransmitter Gonadotropin-Releasing Hormone
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Jones S W
Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106.
References (39)
39 references, click to expand
  1. Chicken II luteinizing hormone-releasing hormone inhibits the M-current of bullfrog sympathetic neurons.
    Neurosci Lett. 1987 Sep 23;80(2):180-4 PMID: 3317136
  2. On the resting potential of isolated frog sympathetic neurons.
    Neuron. 1989 Aug;3(2):153-61 PMID: 2560389
  3. Dihydropyridine actions on calcium currents of frog sympathetic neurons.
    J Neurosci. 1990 Jul;10(7):2261-7 PMID: 1695946
  4. Inhibition of Ca2+ and K+ channels in sympathetic neurons by neuropeptides and other ganglionic transmitters.
    Neuron. 1990 Mar;4(3):379-91 PMID: 1690565
  5. LHRH and GTP-gamma-S modify calcium current activation in bullfrog sympathetic neurons.
    Neuron. 1990 Jul;5(1):75-80 PMID: 2164405
  6. Peptidergic and muscarinic excitation at amphibian sympathetic synapses.
    J Physiol. 1983 Aug;341:257-78 PMID: 6137560
  7. Peptidergic transmission in sympathetic ganglia of the frog.
    J Physiol. 1982 Jun;327:219-46 PMID: 6181250
  8. Pharmacological inhibition of the M-current.
    J Physiol. 1982 Nov;332:223-62 PMID: 6760380
  9. Synaptic inhibition of the M-current: slow excitatory post-synaptic potential mechanism in bullfrog sympathetic neurones.
    J Physiol. 1982 Nov;332:263-72 PMID: 6984074
  10. Voltage-clamp analysis of peptidergic slow depolarizations in bullfrog sympathetic ganglion cells.
    J Physiol. 1982 Dec;333:305-13 PMID: 6763627
  11. A photoisomerizable muscarinic antagonist. Studies of binding and of conductance relaxations in frog heart.
    J Gen Physiol. 1982 Apr;79(4):657-78 PMID: 6978380
  12. Substance P inhibits the M-current in bullfrog sympathetic neurones.
    Br J Pharmacol. 1983 Jun;79(2):330-3 PMID: 6197122
  13. Time course of the increase in the myocardial slow inward current after a photochemically generated concentration jump of intracellular cAMP.
    Proc Natl Acad Sci U S A. 1983 Apr;80(8):2395-9 PMID: 6300914
  14. Single Ca2+-activated nonselective cation channels in neuroblastoma.
    Nature. 1982 Mar 25;296(5855):357-9 PMID: 6278324
  15. Teleost luteinizing hormone-releasing hormone: action on bullfrog sympathetic ganglia is consistent with role as neurotransmitter.
    J Neurosci. 1984 Feb;4(2):420-9 PMID: 6366152
  16. The time course of muscarinic depolarization of guinea-pig myenteric neurones.
    Br J Pharmacol. 1984 May;82(1):85-91 PMID: 6733359
  17. Synaptic delay in the heart: an ionophoretic study.
    J Physiol. 1978 Jun;279:31-54 PMID: 209176
  18. Synaptic excitation and inhibition resulting from direct action of acetylcholine on two types of chemoreceptors on individual amphibian parasympathetic neurones.
    J Physiol. 1977 Oct;271(3):817-46 PMID: 200739
  19. Production of membrane potential changes in the frog's heart by inhibitory nerve impulses.
    Nature. 1955 Jun 11;175(4467):1035 PMID: 14394097
  20. The Influence of Temperature and of Endocardiac Pressure on the Heart, and perticularly on the Action of the Vagus and Cardiac Sympathetic Nerves.
    J Physiol. 1892 Feb;13(1-2):59-164.16 PMID: 16992013
  21. Synaptic events in sympathetic ganglia.
    Prog Neurobiol. 1978;11(2):77-169 PMID: 33420
  22. Effects of muscarine and adrenaline on neurones from Rana pipiens sympathetic ganglia.
    J Physiol. 1990 Jun;425:471-500 PMID: 2213586
  23. Intracellular Ca2+ buffers disrupt muscarinic suppression of Ca2+ current and M current in rat sympathetic neurons.
    Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):652-6 PMID: 1846449
  24. On the transduction mechanism for muscarine-induced inhibition of M-current in cultured rat sympathetic neurones.
    J Physiol. 1989 Jun;413:469-88 PMID: 2689633
  25. Sodium currents in dissociated bull-frog sympathetic neurones.
    J Physiol. 1987 Aug;389:605-27 PMID: 2445980
  26. Alpha-adrenergic inhibition of sympathetic neurotransmitter release mediated by modulation of N-type calcium-channel gating.
    Nature. 1989 Aug 24;340(6235):639-42 PMID: 2570354
  27. Elementary properties and pharmacological sensitivities of calcium channels in mammalian peripheral neurons.
    Neuron. 1989 May;2(5):1453-63 PMID: 2560643
  28. Mechanism of muscarinic receptor-induced K+ channel activation as revealed by hydrolysis-resistant GTP analogues.
    J Gen Physiol. 1988 Apr;91(4):469-93 PMID: 2455765
  29. Muscarine and t-LHRH suppress M-current by activating an IAP-insensitive G-protein.
    J Neurosci. 1988 Sep;8(9):3343-53 PMID: 3139847
  30. The coupling of neurotransmitter receptors to ion channels in the brain.
    Science. 1988 Jul 29;241(4865):545-51 PMID: 2456612
  31. Agonists that suppress M-current elicit phosphoinositide turnover and Ca2+ transients, but these events do not explain M-current suppression.
    Neuron. 1988 Aug;1(6):477-84 PMID: 2483099
  32. Calcium currents in bullfrog sympathetic neurons. I. Activation kinetics and pharmacology.
    J Gen Physiol. 1989 Jul;94(1):151-67 PMID: 2478659
  33. Protein kinase C is not necessary for peptide-induced suppression of M current or for desensitization of the peptide receptors.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2943-7 PMID: 2468164
  34. Adrenaline depolarization in paravertebral sympathetic neurones of bullfrogs.
    Pflugers Arch. 1988 Jan;411(1):80-7 PMID: 2451215
  35. Effects of phorbol dibutyrate on M currents and M current inhibition in bullfrog sympathetic neurons.
    Cell Mol Neurobiol. 1987 Sep;7(3):255-69 PMID: 2449965
  36. Three types of neuronal calcium channel with different calcium agonist sensitivity.
    Nature. 1985 Aug 1-7;316(6027):440-3 PMID: 2410796
  37. Neurotransmitter inhibition of neuronal calcium currents by changes in channel voltage dependence.
    Nature. 1989 Jul 13;340(6229):153-6 PMID: 2567963
  38. Calcium currents in bullfrog sympathetic neurons. II. Inactivation.
    J Gen Physiol. 1989 Jul;94(1):169-82 PMID: 2553857
  39. Ionic channels and their regulation by G protein subunits.
    Annu Rev Physiol. 1990;52:197-213 PMID: 1691904
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(91)82077-X
Published
1991-08-00
Pages
502-7
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1260088
Subset
IM
Grants
NINDS NIH HHS · NS24471 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(91)82077-X PMC Full Text View journal details
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