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PMID: 17172353 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An integrated view of gene expression and solute profiles of Arabidopsis tumors: a genome-wide approach.

The Plant cell ·Vol. 18 ·No. 12 ·2006-12-00 ·Pages 3617-34

Deeken R, Engelmann JC, Efetova M, Czirjak T, Müller T, Kaiser WM, Tietz O, Krischke M, Mueller MJ, Palme K, Dandekar T, Hedrich R

Abstract

Transformation of plant cells with T-DNA of virulent agrobacteria is one of the most extreme triggers of developmental changes in higher plants. For rapid growth and development of resulting tumors, specific changes in the gene expression profile and metabolic adaptations are required. Increased transport and metabolic fluxes are critical preconditions for growth and tumor development. A functional genomics approach, using the Affymetrix whole genome microarray (approximately 22,800 genes), was applied to measure changes in gene expression. The solute pattern of Arabidopsis thaliana tumors and uninfected plant tissues was compared with the respective gene expression profile. Increased levels of anions, sugars, and amino acids were correlated with changes in the gene expression of specific enzymes and solute transporters. The expression profile of genes pivotal for energy metabolism, such as those involved in photosynthesis, mitochondrial electron transport, and fermentation, suggested that tumors produce C and N compounds heterotrophically and gain energy mainly anaerobically. Thus, understanding of gene-to-metabolite networks in plant tumors promotes the identification of mechanisms that control tumor development.

MeSH Terms
Arabidopsis/cytology,genetics,microbiology Arginine/analogs & derivatives,chemistry,metabolism Carbohydrate Metabolism/genetics Cell Wall/metabolism Cluster Analysis Computational Biology DNA, Bacterial/metabolism Energy Metabolism/genetics Gene Expression Profiling Gene Expression Regulation, Plant Genome, Plant/genetics Genomics Lipid Metabolism/genetics Nitrate Reductase/metabolism Nitrogen/metabolism Oligonucleotide Array Sequence Analysis Photosynthesis/genetics Plant Diseases/genetics,microbiology Plant Proteins/genetics RNA, Messenger/genetics,metabolism Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Rhizobium
Chemicals
DNA, Bacterial Plant Proteins RNA, Messenger T-DNA nopaline Arginine Nitrate Reductase Nitrogen
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Deeken Rosalia
Julius-von-Sachs-Institute, Department of Molecular Plant Physiology and Biophysics, University of Wuerzburg, D-97082 Wuerzburg, Germany.
Engelmann Julia C
Efetova Marina
Czirjak Tina
Müller Tobias
Kaiser Werner M
Tietz Olaf
Krischke Markus
Mueller Martin J
Palme Klaus
Dandekar Thomas
Hedrich Rainer
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.106.044743
Published
2006-12-00
Epub
2006-00-15
Pages
3617-34
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1785400
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.106.044743 PMC Full Text View journal details
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