Home LiteratureArticle Details
PMID: 1717995 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

A receptor tyrosine kinase cDNA isolated from a population of enriched primitive hematopoietic cells and exhibiting close genetic linkage to c-kit.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 20 ·1991-10-15 ·Pages 9026-30

Matthews W, Jordan CT, Gavin M, Jenkins NA, Copeland NG, Lemischka IR

Abstract

We have cloned a receptor tyrosine kinase cDNA, designated fetal liver kinase 1 (Flk-1), from mouse cell populations enriched for hematopoietic stem and progenitor cells. Sequence analysis of this clone reveals strong homology to the c-Kit subfamily of receptor kinases, and in particular to the Flt gene product. Chromosomal mapping shows that the Flk-1, Kit, and Pdgfra genes are closely linked. Flk-1 mRNA is expressed in primitive and more mature hematopoietic cells as well as in a wide variety of nonhematopoietic tissues.

Related Genes
Flk-1 Flt Kit Pdgfra c-kit
MeSH Terms
Amino Acid Sequence Animals Base Sequence Chromosome Mapping Crosses, Genetic DNA/genetics,isolation & purification Female Fetus Gene Expression Genetic Linkage Hematopoietic Stem Cells/enzymology Liver/physiology Male Mice Mice, Inbred C57BL Molecular Sequence Data Multigene Family Muridae Polymerase Chain Reaction Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-kit Proto-Oncogenes RNA, Messenger/genetics Receptors, Cell Surface/genetics Sequence Homology, Nucleic Acid
Chemicals
Proto-Oncogene Proteins RNA, Messenger Receptors, Cell Surface DNA Protein-Tyrosine Kinases Proto-Oncogene Proteins c-kit
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Matthews W
Department of Molecular Biology, Princeton University, NJ 08544.
Jordan C T
Gavin M
Jenkins N A
Copeland N G
Lemischka I R
References (26)
26 references, click to expand
  1. Platelet-derived growth factor receptor alpha-subunit gene (Pdgfra) is deleted in the mouse patch (Ph) mutation.
    Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):6-10 PMID: 1846043
  2. Patterns of amino acids near signal-sequence cleavage sites.
    Eur J Biochem. 1983 Jun 1;133(1):17-21 PMID: 6852022
  3. Developmental expression of c-kit, a proto-oncogene encoded by the W locus.
    Development. 1990 Aug;109(4):911-23 PMID: 1699718
  4. A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations.
    Cell. 1991 Jun 28;65(7):1143-52 PMID: 1648448
  5. Development and applications of a molecular genetic linkage map of the mouse genome.
    Trends Genet. 1991 Apr;7(4):113-8 PMID: 2068781
  6. Receptor tyrosine kinases: genetic evidence for their role in Drosophila and mouse development.
    Trends Genet. 1990 Nov;6(11):350-6 PMID: 1965067
  7. Steel locus defines new multipotent growth factor.
    Cell. 1990 Oct 5;63(1):5-6 PMID: 2208282
  8. Expression of c-kit gene products in known cellular targets of W mutations in normal and W mutant mice--evidence for an impaired c-kit kinase in mutant mice.
    Genes Dev. 1989 Jun;3(6):816-26 PMID: 2473008
  9. Isolation of a novel receptor cDNA establishes the existence of two PDGF receptor genes.
    Science. 1989 Feb 10;243(4892):800-4 PMID: 2536956
  10. The dominant-white spotting (W) locus of the mouse encodes the c-kit proto-oncogene.
    Cell. 1988 Oct 7;55(1):185-92 PMID: 2458842
  11. The proto-oncogene c-kit encoding a transmembrane tyrosine kinase receptor maps to the mouse W locus.
    Nature. 1988 Sep 1;335(6185):88-9 PMID: 2457811
  12. A new family of mouse homeo box-containing genes: molecular structure, chromosomal location, and developmental expression of Hox-7.1.
    Genes Dev. 1989 Jan;3(1):26-37 PMID: 2565278
  13. Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction.
    Proc Natl Acad Sci U S A. 1989 Mar;86(5):1603-7 PMID: 2466296
  14. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
    Science. 1988 Jul 1;241(4861):42-52 PMID: 3291115
  15. The immunoglobulin superfamily--domains for cell surface recognition.
    Annu Rev Immunol. 1988;6:381-405 PMID: 3289571
  16. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002 PMID: 2461560
  17. Purification and characterization of mouse hematopoietic stem cells.
    Science. 1988 Jul 1;241(4861):58-62 PMID: 2898810
  18. Growth and differentiation in the hemopoietic system.
    Annu Rev Cell Biol. 1987;3:423-41 PMID: 3318881
  19. Enrichment of hematopoietic precursor cells and cloning of multipotential B-lymphocyte precursors.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7414-8 PMID: 3933007
  20. Signal transduction by receptors with tyrosine kinase activity.
    Cell. 1990 Apr 20;61(2):203-12 PMID: 2158859
  21. Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flt) closely related to the fms family.
    Oncogene. 1990 Apr;5(4):519-24 PMID: 2158038
  22. A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells.
    Cell. 1990 Sep 7;62(5):863-74 PMID: 1975515
  23. Cellular and developmental properties of fetal hematopoietic stem cells.
    Cell. 1990 Jun 15;61(6):953-63 PMID: 1972037
  24. Organization, distribution, and stability of endogenous ecotropic murine leukemia virus DNA sequences in chromosomes of Mus musculus.
    J Virol. 1982 Jul;43(1):26-36 PMID: 6287001
  25. Compilation of published signal sequences.
    Nucleic Acids Res. 1984 Jul 11;12(13):5145-64 PMID: 6379599
  26. Oncogenes and signal transduction.
    Cell. 1991 Jan 25;64(2):281-302 PMID: 1846320
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.20.9026
Published
1991-10-15
Pages
9026-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52644
Subset
IM
Grants
NCI NIH HHS · CA45339 · United States
NIDDK NIH HHS · DK42989 · United States
NCI NIH HHS · N01-CO-74101 · United States
Databases
GENBANK
S60062, S60067, S60069, S60071, S60073, S60076, S60079, S60081, S60382, X59397
External Links
PubMed source DOI: 10.1073/pnas.88.20.9026 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2