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PMID: 1718947 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The Staphylococcus aureus mec determinant comprises an unusual cluster of direct repeats and codes for a gene product similar to the Escherichia coli sn-glycerophosphoryl diester phosphodiesterase.

Journal of bacteriology ·Vol. 173 ·No. 23 ·1991-12-00 ·Pages 7416-22

Ryffel C, Bucher R, Kayser FH, Berger-Bächi B

Abstract

The DNA sequence located between mecA, the gene that codes for penicillin-binding protein PBP2', and insertion sequence-like element IS431mec has been termed hypervariable because of its length polymorphism among different staphylococcal isolates. We sequenced and characterized the hypervariable region of the methicillin resistance determinant (mec) isolated from Staphylococcus aureus BB270. Within the 2,040-bp hypervariable region, we identified an unusual accumulation of long direct repeats. Analysis of the DNA sequence revealed a minimal direct repeat unit (dru) of 40 bp which was repeated 10 times within 500 bp. The dru sequences are responsible for the length polymorphism of mec. Moreover, we identified an open reading frame that codes for 145 amino acids (ORF145), whose deduced amino acid sequence showed 57% amino acid sequence similarity to the N terminus of the glycerophosphoryl diester phosphodiesterase (UgpQ) of Escherichia coli.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Bacterial/genetics,isolation & purification Escherichia coli/enzymology,genetics Genes, Bacterial Genetic Variation Molecular Sequence Data Multigene Family Open Reading Frames Phosphoric Diester Hydrolases/genetics Plasmids RNA, Bacterial/genetics,isolation & purification Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Staphylococcus aureus/enzymology,genetics
Chemicals
DNA, Bacterial RNA, Bacterial Phosphoric Diester Hydrolases glycerophosphodiester phosphodiesterase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ryffel C
Institute of Medical Microbiology, University of Zurich, Switzerland.
Bucher R
Kayser F H
Berger-Bächi B
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.23.7416-7422.1991
Published
1991-12-00
Pages
7416-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212504
Subset
IM
Databases
GENBANK
M63255, M64328, M64329, M64330, M64331, M64332, M76658, S64936, S64939, S65229, X52594
External Links
PubMed source DOI: 10.1128/jb.173.23.7416-7422.1991 PMC Full Text View journal details
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