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PMID: 17194703 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Chlamydomonas flagellar outer row dynein assembly protein ODA7 interacts with both outer row and I1 inner row dyneins.

The Journal of biological chemistry ·Vol. 282 ·No. 8 ·2007-02-23 ·Pages 5404-12

Freshour J, Yokoyama R, Mitchell DR

Abstract

We previously found that a mutation at the ODA7 locus in Chlamydomonas prevents axonemal outer row dynein assembly by blocking association of heavy chains and intermediate chains in the cytoplasm. We have now cloned the ODA7 locus by walking in the Chlamydomonas genome from nearby molecular markers, confirmed the identity of the gene by rescuing the mutant phenotype with genomic clones, and identified the ODA7 gene product as a 58-kDa leucine-rich repeat protein unrelated to outer row dynein LC1. Oda7p is missing from oda7 mutant flagella but is present in flagella of other outer row or inner row dynein assembly mutants. However, Oda7 levels are greatly reduced in flagella that lack both outer row dynein and inner row I1 dynein. Biochemical fractionation and rebinding studies support a model in which Oda7 participates in a previously uncharacterized structural link between inner and outer row dyneins.

MeSH Terms
Algal Proteins/genetics,metabolism Animals Chlamydomonas reinhardtii/genetics,metabolism,ultrastructure Cloning, Molecular Dyneins/genetics,metabolism Flagella/genetics,metabolism,ultrastructure Genome, Plant/physiology Genome, Protozoan/physiology Models, Biological Mutation Protozoan Proteins/genetics,metabolism Quantitative Trait Loci/physiology
Chemicals
Algal Proteins Protozoan Proteins Dyneins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Freshour Judy
Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA.
Yokoyama Ruth
Mitchell David R
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
DOI
10.1074/jbc.M607509200
Published
2007-02-23
Epub
2006-00-27
Pages
5404-12
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC3321484
Subset
IM
Grants
NIGMS NIH HHS · R01 GM044228 · United States
NIGMS NIH HHS · R01 GM044228-15 · United States
NIGMS NIH HHS · GM44228 · United States
External Links
PubMed source DOI: 10.1074/jbc.M607509200 PMC Full Text View journal details
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