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PMID: 1720861 Published · ppublish English Journal Article

Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 230 ·No. 1-2 ·1991-11-00 ·Pages 230-40

Klein JR, Henrich B, Plapp R

Abstract

The chromosomal DNA insert in plasmid pJK131, which complements the phenotypic defects associated with a mutation in the envC gene of Escherichia coli strain PM61, was sequenced. The analysis of the nucleotide sequence revealed two open reading frames (ORFs) coding for the proteins EnvC (41,281 daltons) and EnvD (104,415 daltons). The envC gene product is synthesized as a pre-protein and, after cleavage of a signal peptide, the mature protein is incorporated into the cytoplasmic membrane. The detection of a common transcript for both ORFs indicated the existence of an envCD operon. Deletion analysis and the generation of frameshifts demonstrated that simultaneous expression of both genes is required to complement the defects in strain PM61. Overproduction of EnvC protein appears to be lethal to Escherichia coli. The envD gene, however, could be cloned and expressed at high levels under control of the tac promoter without deleterious effects on the host.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Base Sequence Blotting, Northern Carrier Proteins Cell Membrane/metabolism DNA, Bacterial/genetics Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Escherichia coli Proteins Frameshift Mutation Genes, Bacterial Membrane Proteins/genetics,metabolism Molecular Sequence Data Multidrug Resistance-Associated Proteins Open Reading Frames Operon Plasmids Promoter Regions, Genetic RNA, Bacterial/genetics Restriction Mapping Transcription, Genetic
Chemicals
AcrB protein, E coli Bacterial Proteins Carrier Proteins DNA, Bacterial Escherichia coli Proteins Membrane Proteins Multidrug Resistance-Associated Proteins RNA, Bacterial acrF protein, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Klein J R
Abteilung Mikrobiologie, Universität Kaiserslautern, FRG.
Henrich B
Plapp R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00290673
Published
1991-11-00
Pages
230-40
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
S55527, S55528, S55529, S55530, S55531, S55532, S63909, S69884, S69885, S70446, X57948
External Links
PubMed source DOI: 10.1007/BF00290673
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