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PMID: 1720891 Published · ppublish English Journal Article

The inhibitory action of caffeine on calcium currents in isolated intestinal smooth muscle cells.

Pflugers Archiv : European journal of physiology ·Vol. 419 ·No. 3-4 ·1991-10-00 ·Pages 267-73

Zholos AV, Baidan LV, Shuba MF

Abstract

The patch-clamp method has been used to investigate the action of caffeine on the calcium current (ICa) in single isolated smooth muscle cells of the guinea-pig ileum. Caffeine (10 mM) substantially inhibited ICa. This effect occurred in a biphasic manner and it was not due either to activation of additional ionic currents of opposite direction nor to inhibition of phosphodiesterase activity. It strongly depended upon the ethylenebis-(oxonitrilo)tetraacetate (EGTA) concentration in the pipette solution. When there was K+ in the pipette solution, application of caffeine evoked a transient Ca-dependent K+ current and an abrupt and transient increase in the frequency of channel openings. Such well-known blockers of Ca release as procaine and ruthenium red strongly decreased ICa. Ryanodine had only little effect on ICa, but application of caffeine in the presence of ryanodine led to a complete and irreversible inhibition of ICa. The results of experiments involving different EGTA concentrations and comparison of the time courses of all caffeine-induced phenomena clearly indicated that only the initial, transient component of the ICa inhibition by caffeine was related to a Ca-dependent inactivation of Ca channels, evoked as a result of Ca release from intracellular stores. The tonic component of ICa inhibition was probably due to a direct blocking action of caffeine on Ca channels.

MeSH Terms
1-Methyl-3-isobutylxanthine/pharmacology Animals Caffeine/pharmacology Calcium/pharmacology Calcium Channel Blockers/pharmacology Calcium Channels/drug effects,physiology Cell Separation Electrophysiology Intestinal Mucosa/metabolism Intestines/cytology Muscle, Smooth/cytology,metabolism Potassium Channels/drug effects,physiology Time Factors
Chemicals
Calcium Channel Blockers Calcium Channels Potassium Channels Caffeine Calcium 1-Methyl-3-isobutylxanthine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zholos A V
Department of Nerve-Muscle Physiology, Bogomoletz Institute of Physiology, Kiev, USSR.
Baidan L V
Shuba M F
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00371106
Published
1991-10-00
Pages
267-73
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00371106 View journal details
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