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PMID: 172448 Published · ppublish English Journal Article

Murine cytomegalovirus: induction of and sensitivity to interferon in vitro.

Infection and immunity ·Vol. 12 ·No. 5 ·1975-11-00 ·Pages 1012-7

Oie HK, Easton JM, Ablashi DV, Baron S

Abstract

Moderate amounts of viral inhibitor were produced by mouse embryo (ME) cultures infected with two strains of plaque-purified murine cytomegalovirus (MCV). This inhibitor was shown to be interferon, based on the possession of similar properties. The growth studies of MCV in ME cells showed that interferon was produced as early as 4 h after infection, infectious virus was produced between 12 to 16 h, and cytopathic effect was produced between 16 to 18 h. Since MCV-induced interferon production and the subsequent development of antiviral state occurred early, the long eclipse period may be due to an interferon-mediated delay of virus replication. Pretreatment of ME cells with varying concentrations of interferon before infection with MCV did not result in increased interferon production, but at high pretreatment doses a slight inhibitory effect on interferon production was observed. In vitro sensitivity studies showed that small doses of MCV were highly sensitive to the antiviral action of interferon, but higher viral doses proved to be markedly resistant. Although the available evidence does not permit a definitive interpretation of the mechanism by which MCV may show differing sensitivities to interferon action, the presence of a small interferon-resistant fraction of virus-infected cells may account for the observations.

MeSH Terms
Animals Chick Embryo Cytomegalovirus/metabolism Cytopathogenic Effect, Viral In Vitro Techniques Interferons/analysis,biosynthesis,pharmacology Mice Sindbis Virus/metabolism Time Factors
Chemicals
Interferons
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Oie H K
Easton J M
Ablashi D V
Baron S
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32 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.12.5.1012-1017.1975
Published
1975-11-00
Pages
1012-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC415390
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.12.5.1012-1017.1975 PMC Full Text View journal details
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