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PMID: 17248770 Published · ppublish English Journal Article

Generalized Transduction in CAULOBACTER CRESCENTUS.

Genetics ·Vol. 87 ·No. 3 ·1977-11-00 ·Pages 391-9

Ely B, Johnson RC

Abstract

Two closely related bacteriophage, varphiCr30 and varphiCr35, are the first bacteriophage shown to mediate generalized transduction in Caulobacter crescentus. Unlike most other transducing phage, they are virulent and do not form any sort of lysogenic relationship with their host. However, they are rather inefficient at adsorption, so that transductants have a good chance of survival. The phage particles have a head 80 nm in diameter and a contractile tail 140 nm in length. Procedures for growth and transduction with varphiCr30 are relatively simple; thus, it will be of great value for the genetic analysis of C. crescentus.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ely B
Biology Department, University of South Carolina, Columbia, South Carolina 29208.
Johnson R C
References (12)
12 references, click to expand
  1. Isolation of spontaneously derived mutants of Caulobacter crescentus.
    Genetics. 1977 May;86(1):25-32 PMID: 407126
  2. Specific cleavage analysis of mammalian mitochondrial DNA.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4496-500 PMID: 1060130
  3. Gene transfer in Caulobacter crescentus: polarized inheritance of genetic markers.
    Genetics. 1975 May;80(1):1-11 PMID: 1137965
  4. Polarity of gene transfer in Caulobacter.
    J Gen Microbiol. 1975 Nov;91(1):183-7 PMID: 1202158
  5. Isolation and characterization of Caulobacter crecentus bacteriophage phi Cd1.
    J Virol. 1976 Feb;17(2):568-75 PMID: 1255848
  6. ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGES ACTIVE AGAINST STALKED BACTERIA.
    J Gen Microbiol. 1965 Apr;39:95-107 PMID: 14328417
  7. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  8. Some improved methods in P22 transduction.
    Genetics. 1974 Apr;76(4):625-31 PMID: 4599954
  9. Plasmid identification using specific endonucleases.
    Mol Gen Genet. 1974;133(2):141-9 PMID: 4614064
  10. Transduction by bacteriophage T1.
    Proc Natl Acad Sci U S A. 1970 Aug;66(4):1083-8 PMID: 4920089
  11. Bacterial differentiation.
    Science. 1971 Sep 3;173(4000):884-92 PMID: 5572165
  12. Transduction in Bacillus subtilis by bacteriophage SPP1.
    J Virol. 1974 Dec;14(6):1343-8 PMID: 4214946
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/87.3.391
Published
1977-11-00
Pages
391-9
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1213749
External Links
PubMed source DOI: 10.1093/genetics/87.3.391 PMC Full Text View journal details
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