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PMID: 17248995 Published · ppublish English Journal Article

Effects of the RAD52 Gene on Recombination in SACCHAROMYCES CEREVISIAE.

Genetics ·Vol. 94 ·No. 1 ·1980-01-00 ·Pages 31-50

Prakash S, Prakash L, Burke W, Montelone BA

Abstract

Effects of the rad52 mutation in Saccharomyces cerevisiae on meiotic, gamma-ray-induced, UV-induced and spontaneous mitotic recombination were studied. The rad52/rad52 diploids undergo premeiotic DNA synthesis; sporulation occurs but inviable spores are produced. Both intra and intergenic recombination during meiosis were examined in cells transferred from sporulation medium to vegetative medium at different time intervals. No intragenic recombination was observed at the his1-1/his1-315 and trp5-2/trp5-48 heteroalleles. Gene-centromere recombination also was not observed in rad52/rad52 diploids. No gamma-ray- or UV-induced intragenic mitotic recombination is seen in rad52/rad52 diploids. The rate of spontaneous mitotic recombination is lowered five-fold at the his1-1/his1-315 and leu1-c/leu1-12 heteroalleles. Spontaneous reversion rates of both his1-1 and his1-315 were elevated 10 to 20 fold in rad52/rad52 diploids.-The RAD52 gene function is required for spontaneous mitotic recombination, UV- and gamma-ray-induced mitotic recombination and meiotic recombination.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Prakash S
Department of Biology, University of Rochester, Rochester, New York 14627.
Prakash L
Burke W
Montelone B A
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/94.1.31
Published
1980-01-00
Pages
31-50
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1214136
External Links
PubMed source DOI: 10.1093/genetics/94.1.31 PMC Full Text View journal details
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