Home LiteratureArticle Details
PMID: 1727490 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The region of the envelope gene of human immunodeficiency virus type 1 responsible for determination of cell tropism.

Journal of virology ·Vol. 66 ·No. 1 ·1992-01-00 ·Pages 305-9

Cann AJ, Churcher MJ, Boyd M, O'Brien W, Zhao JQ, Zack J, Chen IS

Abstract

Different isolates of human immunodeficiency virus type 1 (HIV-1) vary in the cell tropisms they display, i.e., the range of cell types in which they are able to establish a productive infection. Here, we report on the phenotypes of recombinants between two molecularly cloned strains of HIV-1. Our results prove that the envelope glycoprotein gp120 is solely responsible for the difference in cell tropism between the two parental isolates and that no other genes or sequences are involved in determining the cell tropism of these strains. The region of the envelope involved in the determination of cell tropism includes sequences which encode the V3 loop of gp120. Control of cell tropism by this region of the virus env gene is a general phenomenon which applies to many different HIV-1 isolates.

MeSH Terms
Amino Acid Sequence Cell Line Cells, Cultured Cloning, Molecular HIV Envelope Protein gp120/chemistry,genetics,metabolism HIV-1/genetics,growth & development Humans Molecular Sequence Data Polymerase Chain Reaction Receptors, Virus/metabolism Sequence Alignment
Chemicals
HIV Envelope Protein gp120 Receptors, Virus
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Cann A J
Department of Microbiology, University of Leicester, United Kingdom.
Churcher M J
Boyd M
O'Brien W
Zhao J Q
Zack J
Chen I S
References (33)
33 references, click to expand
  1. CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA.
    Cancer. 1965 Apr;18:522-9 PMID: 14278051
  2. Distinct replicative and cytopathic characteristics of human immunodeficiency virus isolates.
    J Virol. 1988 Nov;62(11):4414-9 PMID: 2459416
  3. The V3 loops of the HIV-1 and HIV-2 surface glycoproteins contain proteolytic cleavage sites: a possible function in viral fusion?
    AIDS Res Hum Retroviruses. 1991 Jan;7(1):3-16 PMID: 2015114
  4. The long terminal repeat is not a major determinant of the cellular tropism of human immunodeficiency virus type 1.
    J Virol. 1991 Feb;65(2):1041-5 PMID: 1987367
  5. Identification of the envelope V3 loop as the primary determinant of cell tropism in HIV-1.
    Science. 1991 Jul 5;253(5015):71-4 PMID: 1905842
  6. Macrophage and T cell-line tropisms of HIV-1 are determined by specific regions of the envelope gp120 gene.
    Nature. 1991 Jan 10;349(6305):167-9 PMID: 1986308
  7. Factors affecting cellular tropism of human immunodeficiency virus.
    J Virol. 1990 Nov;64(11):5600-4 PMID: 1976823
  8. HIV-1 tropism for mononuclear phagocytes can be determined by regions of gp120 outside the CD4-binding domain.
    Nature. 1990 Nov 1;348(6296):69-73 PMID: 2172833
  9. Differences in cytopathogenicity and host cell range among infectious molecular clones of human immunodeficiency virus type 1 simultaneously isolated from an individual.
    J Virol. 1988 Nov;62(11):4078-85 PMID: 3172338
  10. Effects of mutations in hyperconserved regions of the extracellular glycoprotein of human immunodeficiency virus type 1 on receptor binding.
    J Virol. 1989 Oct;63(10):4464-8 PMID: 2550679
  11. Expression of membrane-associated and secreted variants of gp160 of human immunodeficiency virus type 1 in vitro and in continuous cell lines.
    J Virol. 1988 Sep;62(9):3135-42 PMID: 2841466
  12. Delineation of a region of the human immunodeficiency virus type 1 gp120 glycoprotein critical for interaction with the CD4 receptor.
    Cell. 1987 Sep 11;50(6):975-85 PMID: 2441877
  13. Evidence for a role of virulent human immunodeficiency virus (HIV) variants in the pathogenesis of acquired immunodeficiency syndrome: studies on sequential HIV isolates.
    J Virol. 1989 May;63(5):2118-25 PMID: 2564898
  14. In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity.
    J Virol. 1988 Jan;62(1):139-47 PMID: 3257102
  15. Detection of a fusion peptide sequence in the transmembrane protein of human immunodeficiency virus.
    Cell. 1987 Jul 31;50(3):327-8 PMID: 3496970
  16. Dual infection of the central nervous system by AIDS viruses with distinct cellular tropisms.
    Science. 1987 May 15;236(4803):819-22 PMID: 3646751
  17. Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1.
    Science. 1987 Sep 11;237(4820):1351-5 PMID: 3629244
  18. Human immunodeficiency virus type 1 T-cell tropism is determined by events prior to provirus formation.
    J Virol. 1990 Oct;64(10):4735-42 PMID: 2398528
  19. Differences in the basal activity of the long terminal repeat determine different replicative capacities of two closely related human immunodeficiency virus type 1 isolates.
    J Virol. 1990 Aug;64(8):3654-60 PMID: 2370677
  20. Viral determinants of human immunodeficiency virus type 1 T-cell or macrophage tropism, cytopathogenicity, and CD4 antigen modulation.
    J Virol. 1990 Sep;64(9):4390-8 PMID: 2384920
  21. Human immunodeficiency virus type 1 cellular host range, replication, and cytopathicity are linked to the envelope region of the viral genome.
    J Virol. 1990 Aug;64(8):4016-20 PMID: 2370688
  22. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.
    Science. 1984 May 4;224(4648):497-500 PMID: 6200935
  23. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas.
    Blood. 1980 Mar;55(3):409-17 PMID: 6244013
  24. Restricted expression of human T-cell leukemia--lymphoma virus (HTLV) in transformed human umbilical cord blood lymphocytes.
    Virology. 1983 Aug;129(1):51-64 PMID: 6412453
  25. A neutralizing epitope of human immunodeficiency virus type 1 has homologous amino acid sequences with the active site of inter-alpha-trypsin inhibitor.
    Int Immunol. 1989;1(6):613-8 PMID: 2484964
  26. Expression and immunogenicity of the extracellular domain of the human immunodeficiency virus type 1 envelope glycoprotein, gp160.
    J Virol. 1989 Aug;63(8):3489-98 PMID: 2545918
  27. Biologic features of HIV-1 that correlate with virulence in the host.
    Science. 1988 Apr 1;240(4848):80-2 PMID: 2832945
  28. The role of mononuclear phagocytes in HTLV-III/LAV infection.
    Science. 1986 Jul 11;233(4760):215-9 PMID: 3014648
  29. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone.
    J Virol. 1986 Aug;59(2):284-91 PMID: 3016298
  30. Structure and function of the HIV envelope.
    AIDS. 1989;3 Suppl 1:S35-41 PMID: 2482055
  31. Single amino-acid changes in HIV envelope affect viral tropism and receptor binding.
    Nature. 1989 Aug 17;340(6234):571-4 PMID: 2475780
  32. Involvement of tryptase-related cellular protease(s) in human immunodeficiency virus type 1 infection.
    FEBS Lett. 1989 May 8;248(1-2):48-52 PMID: 2470618
  33. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.1.305-309.1992
Published
1992-01-00
Pages
305-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC238288
Subset
IM
Grants
NIAID NIH HHS · AI29107 · United States
NINDS NIH HHS · NS25508 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.1.305-309.1992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2