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PMID: 17286820 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Guard cell-specific inhibition of Arabidopsis MPK3 expression causes abnormal stomatal responses to abscisic acid and hydrogen peroxide.

The New phytologist ·Vol. 173 ·No. 4 ·2007-00-00 ·Pages 713-721

Gudesblat GE, Iusem ND, Morris PC

Abstract

MAP kinases have been linked to guard cell signalling. Arabidopsis thaliana MAP Kinase 3 (MPK3) is known to be activated by abscisic acid (ABA) and hydrogen peroxide (H(2)O(2)), which also control stomatal movements. We therefore studied the possible role of MPK3 in guard cell signalling through guard cell-specific antisense inhibition of MPK3 expression. Such transgenic plants contained reduced levels of MPK3 mRNA in the guard cells and displayed partial insensitivity to ABA in inhibition of stomatal opening, but responded normally to this hormone in stomatal closure. However, ABA-induced stomatal closure was reduced compared with controls when cytoplasmic alkalinization was prevented with sodium butyrate. MPK3 antisense plants were less sensitive to exogenous H(2)O(2), both in inhibition of stomatal opening and in promotion of stomatal closure, thus MPK3 is required for the signalling of this compound. ABA-induced H(2)O(2) synthesis was normal in these plants, indicating that MPK3 probably acts in signalling downstream of H(2)O(2). These results provide clear evidence for the important role of MPK3 in the perception of ABA and H(2)O(2) in guard cells.

MeSH Terms
Abscisic Acid/pharmacology Arabidopsis/cytology,physiology Arabidopsis Proteins/metabolism DNA, Antisense/metabolism Hydrogen Peroxide/pharmacology Mitogen-Activated Protein Kinase 3/genetics,metabolism Plant Epidermis/cytology,physiology Plant Leaves/cytology,physiology Plants, Genetically Modified Signal Transduction Transformation, Genetic
Chemicals
Arabidopsis Proteins DNA, Antisense Abscisic Acid Hydrogen Peroxide Mitogen-Activated Protein Kinase 3
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gudesblat Gustavo E
Laboratorio de Fisiología, Biología Molecular y Celular, IFIByNE - CONICET, Facultad de Ciencias Exactas y Naturales - UBA, 1428 Buenos Aires, Argentina. | Instituto Leloir, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina.
Iusem Norberto D
Laboratorio de Fisiología, Biología Molecular y Celular, IFIByNE - CONICET, Facultad de Ciencias Exactas y Naturales - UBA, 1428 Buenos Aires, Argentina.
Morris Peter C
School of Life Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UK.
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Article Info
Journal
The New phytologist
Abbr.
New Phytol
ISSN
0028-646X
DOI
10.1111/j.1469-8137.2006.01953.x
Published
2007-00-00
Pages
713-721
Language
English
Region
England
NLM ID
9882884
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1469-8137.2006.01953.x View journal details
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