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PMID: 17287021 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Drosophila C-type lectins enhance cellular encapsulation.

Molecular immunology ·Vol. 44 ·No. 10 ·2007-04-00 ·Pages 2541-8

Ao J, Ling E, Yu XQ

Abstract

C-type lectins are calcium-dependent carbohydrate binding proteins, and animal C-type lectins participate in innate immunity and cell-cell interactions. In the fruit fly Drosophila melanogaster, more than 30 genes encode C-type lectin domains. However, functions of Drosophila C-type lectins in innate immunity are not well understood. This study is to investigate whether two Drosophila C-type lectins, CG33532 and CG33533 (designated as DL2 and DL3, respectively), are involved in innate immune responses. Recombinant DL2 and DL3 were expressed and purified. Both DL2 and DL3 agglutinated Gram-negative Escherichia coli in a calcium-dependent manner. Though DL2 and DL3 are predicted to be secreted proteins, they were detected on the surface of Drosophila hemocytes, and recombinant DL2 and DL3 also directly bound to hemocytes. Coating of agarose beads with recombinant DL2 and DL3 enhanced their encapsulation and melanization by Drosophila hemocytes in vitro. However, hemocyte encapsulation was blocked when the lectin-coated beads were pre-incubated with rat polyclonal antibody specific for DL2 or DL3. Our results suggest that DL2 and DL3 may act as pattern recognition receptors to mediate hemocyte encapsulation and melanization by directly recruiting hemocytes to the lectin-coated surface.

MeSH Terms
Animals Drosophila Proteins/analysis,genetics,immunology Drosophila melanogaster/immunology Escherichia coli/immunology Hemocytes/chemistry,immunology Immunity, Innate Lectins, C-Type/analysis,genetics,immunology Receptors, Pattern Recognition/genetics,immunology Recombinant Proteins/genetics,immunology
Chemicals
Drosophila Proteins Lectins, C-Type Receptors, Pattern Recognition Recombinant Proteins lectin-37Da protein, Drosophila lectin-37Db protein, Drosophila
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ao Jingqun
Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA.
Ling Erjun
Yu Xiao-Qiang
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Article Info
Journal
Molecular immunology
Abbr.
Mol Immunol
ISSN
0161-5890
DOI
10.1016/j.molimm.2006.12.024
Published
2007-04-00
Epub
2007-00-06
Pages
2541-8
Language
English
Region
England
NLM ID
7905289
PMCID
PMC1876673
Subset
IM
Grants
NIGMS NIH HHS · R01 GM066356 · United States
NIGMS NIH HHS · GM 66356 · United States
External Links
PubMed source DOI: 10.1016/j.molimm.2006.12.024 PMC Full Text View journal details
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