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PMID: 172887 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Partial purification of a glucocorticoid receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 10 ·1975-10-00 ·Pages 3849-52

Failla D, Tomkins GM, Santi DV

Abstract

A simple method for purification of the glucocorticoid receptor from hepatoma tissue culture cells has been developed. The procedure, which requires only about 24 hr, involves biospecific adsorption of the receptor to deoxycorticosterone derivatized agarose, elution with a glucocorticoid, and gel filtration. The receptor-steroid complex is obtained in 35-40% yield and is about 2000-fold purified. It possesses properties similar to those reported in crude extracts, including sedimentation coefficient in glycerol gradients and activation-dependent binding to nuclei.

MeSH Terms
Binding Sites Carcinoma, Hepatocellular/analysis,metabolism Cell Line Chromatography, Affinity Dexamethasone/metabolism Glucocorticoids/metabolism Liver Neoplasms Molecular Weight Neoplasm Proteins/isolation & purification,metabolism Protein Binding Receptors, Cell Surface Tetrahydrocortisol/metabolism Triamcinolone Acetonide/metabolism
Chemicals
Glucocorticoids Neoplasm Proteins Receptors, Cell Surface Tetrahydrocortisol Dexamethasone Triamcinolone Acetonide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Failla D
Tomkins G M
Santi D V
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.10.3849
Published
1975-10-00
Pages
3849-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433093
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.10.3849 PMC Full Text View journal details
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