Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage T4 endonuclease V and HVJ (Sendai virus).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 10 ·1975-10-00 ·Pages 4071-5

Tanaka K, Sekiguchi M, Okada Y

Abstract

Ultraviolet (UV)-induced unscheduled DNA synthesis of xeroderma pigmentosum cells, belonging to complementation groups A, B, C, D, and E, was restored to the normal level by concomitant treatment of the cells with T4 endonuclease V and UV-inactivated HVJ (Sendai virus). The present results suggest that (1) T4 endonuclease molecules were inserted effectively into the cells by the interaction of HVJ with the cell membranes, (2) the enzyme was functional on human chromosomal DNA which had been damaged by UV irradiation in the viable cells, (3) all the studied groups of xeroderma pigmentosum ("variant" was not tested) were defective in the first step (incision) of excision repair.

MeSH Terms

Cells, Cultured Coliphages/enzymology DNA Replication/radiation effects DNA, Neoplasm/radiation effects Deoxyribonucleases/metabolism Endonucleases/metabolism Humans Parainfluenza Virus 1, Human Radiation Effects Ultraviolet Rays Xeroderma Pigmentosum/metabolism

Chemicals

DNA, Neoplasm Deoxyribonucleases Endonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Tanaka K

Sekiguchi M

Okada Y

References (24)

24 references, click to expand

Genetic heterogeneity in xeroderma pigmentosum: complementation groups and their relationship to DNA repair rates.
Proc Natl Acad Sci U S A. 1975 Jan;72(1):59-63 PMID: 164028
Xeroderma pigmentosum cells with normal levels of excision repair have a defect in DNA synthesis after UV-irradiation.
Proc Natl Acad Sci U S A. 1975 Jan;72(1):219-23 PMID: 1054497
Xeroderma pigmentosum variants have decreased repair of ultraviolet-damaged DNA.
Nature. 1975 Feb 27;253(5494):748-9 PMID: 1113871
Different ultraviolet DNA endonuclease activity in human cells.
Nature. 1975 May 1;255(5503):82-4 PMID: 1128675
Requirement of calcium ions for the cell fusion reaction of animal cells by HVJ.
Exp Cell Res. 1966 Nov-Dec;44(2):527-51 PMID: 4290343
A UV-specific endonucleolytic activity present in human cell extracts.
Biochem Biophys Res Commun. 1972 Aug 7;48(3):662-9 PMID: 4340112
Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization.
Nat New Biol. 1972 Jul 19;238(81):80-3 PMID: 4505415
Repair enzyme suggested by mammalian endonuclease activity specific for ultraviolet-irradiated DNA.
Nat New Biol. 1972 Oct 11;239(93):172-3 PMID: 4512840
Induction of a new enzyme activity to excise pyrimidine dimers in Escherichia coli infected with bacteriophage T4.
Biochem Biophys Res Commun. 1972 Jun 9;47(5):1126-32 PMID: 4555248
Initial step of excision repair in Escherichia coli: replacement of defective function of uvr mutants by T4 endonuclease V.
J Mol Biol. 1972 Sep 14;70(1):1-14 PMID: 4561346
An endonuclease from Escherichia coli that acts preferentially on UV-irradiated DNA and is absent from the uvrA and uvrB mutants.
Proc Natl Acad Sci U S A. 1974 May;71(5):1838-42 PMID: 4599989
Use of UV endonuclease from Micrococcus luteus to monitor the progress of DNA repair in UV-irradiated human cells.
Mutat Res. 1973 Aug;19(2):245-56 PMID: 4748980
Repair endonuclease-sensitive sites in daughter DNA of ultraviolet-irradiated human cells.
Nature. 1973 Dec 21-28;246(5434):484 PMID: 4762196
Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4.
J Biol Chem. 1968 Feb 10;243(3):627-38 PMID: 4866523
Nucleases specific for ultraviolet light-irradiated DNA and their possible role in dark repair.
Cold Spring Harb Symp Quant Biol. 1968;33:219-27 PMID: 4891964
Dark repair of ultraviolet-irradiated deoxyribonucleic acid by bacteriophage T4: purification and characterization of a dimer-specific phage-induced endonuclease.
J Bacteriol. 1971 May;106(2):500-7 PMID: 4929862
Xeroderma pigmentosum: variants with normal DNA repair and normal sensitivity to ultraviolet light.
J Invest Dermatol. 1972 Mar;58(3):124-8 PMID: 5013606
Evidence that xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA.
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1035-41 PMID: 5264135
T4 endonuclease involved in repair of DNA.
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1839-45 PMID: 5275382
Defect in DNA synthesis in skin of patients with xeroderma pigmentosum demonstrated in vivo.
Science. 1970 Jun 19;168(3938):1477-8 PMID: 5445941
Defective repair replication of DNA in xeroderma pigmentosum.
Nature. 1968 May 18;218(5142):652-6 PMID: 5655953
Sedimentation of DNA from human fibroblasts irradiated with ultraviolet light: possible detection of excision breaks in normal and repair-deficient xeroderma pigmentosum cells.
Radiat Res. 1974 Feb;57(2):207-27 PMID: 10874937
Analysis of giant polynuclear cell formation caused by HVJ virus from Ehrlich's ascites tumor cells. II. Quantitative analysis of giant polynuclear cell formation.
Exp Cell Res. 1962 Feb;26:108-18 PMID: 14481502
Replication of poliovirus I in chick embryo and hamster cells exposed to sendai virus.
Proc Natl Acad Sci U S A. 1967 Mar;57(3):637-44 PMID: 16591511

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.10.4071

Published

1975-10-00

Pages

4071-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433140

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.10.4071 PMC Full Text View journal details

Analysis Services

Restoration of ultraviolet-induced unscheduled DNA synthesis of xeroderma pigmentosum cells by the concomitant treatment with bacteriophage T4 endonuclease V and HVJ (Sendai virus).

Contact

WeChat Official Account