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PMID: 1729246 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of spoIVA, a sporulation gene involved in coat morphogenesis in Bacillus subtilis.

Journal of bacteriology ·Vol. 174 ·No. 2 ·1992-01-00 ·Pages 575-85

Roels S, Driks A, Losick R

Abstract

We report the cloning and characterization of the Bacillus subtilis sporulation locus spoIVA, mutations at which cause an unusual defect in spore formation in which the coat misassembles as swirls within the mother cell. We show that spoIVA is a single gene of 492 codons that is capable of encoding a polypeptide of 55 kDa. Transcription of spoIVA is induced at about the second hour of sporulation by the regulatory protein sigma E from two closely spaced promoters designated P1 and P2. Experiments in which the upstream promoter P1 was removed show that transcription of spoIVA from P2 is sufficient for efficient spore formation. Based on these and other findings, we infer that the spoIVA gene product is a morphogenetic protein; we discuss its role in the deposition of coat polypeptides around the developing forespore.

Related Genes
spoIVA
MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics,growth & development,ultrastructure Bacterial Proteins/genetics Base Sequence Chromosome Mapping Chromosomes, Bacterial/chemistry Cloning, Molecular DNA, Bacterial/chemistry Genes, Bacterial Lac Operon Molecular Sequence Data Sigma Factor Spores, Bacterial/chemistry,physiology,ultrastructure Transcription Factors Transcription, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial Sigma Factor Transcription Factors spoIIR protein, Bacillus subtilis spore-specific proteins, Bacillus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roels S
Department of Cellular and Developmental Biology Laboratories, Harvard University, Cambridge, Massachusetts 02138.
Driks A
Losick R
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.2.575-585.1992
Published
1992-01-00
Pages
575-85
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205752
Subset
IM
Grants
NIGMS NIH HHS · GM18568 · United States
Databases
GENBANK
M80926, M94060, M94061, M94062, M94628, M94629, X64378, X64379, X64380, X64381
External Links
PubMed source DOI: 10.1128/jb.174.2.575-585.1992 PMC Full Text View journal details
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