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PMID: 1729712 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 1 ·1992-01-01 ·Pages 426-30

Shiraga H, Min W, VanDusen WJ, Clayman MD, Miner D, Terrell CH, Sherbotie JR, Foreman JW, Przysiecki C, Neilson EG

Abstract

The majority of human urinary stones are primarily composed of calcium salts. Although normal urine is frequently supersaturated with respect to calcium oxalate, most humans do not form stones. Inhibitors are among the multiple factors that may influence the complex process of urinary stone formation. We have isolated an inhibitor of calcium oxalate crystal growth from human urine by monoclonal antibody immunoaffinity chromatography. The N-terminal amino acid sequence and acidic amino acid content of this aspartic acid-rich protein, uropontin, are similar to those of other pontin proteins from bone, plasma, breast milk, and cells. The inhibitory effect of uropontin on calcium oxalate crystal growth in vitro supports the concept that pontins may have a regulatory role. This function would be analogous to that of other members of the aspartic acid-rich protein superfamily, which stereospecifically regulate the mineralization fronts of calcium-containing crystals.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal Aspartic Acid/chemistry Calcium Oxalate/chemistry Crystallization Humans Molecular Sequence Data Multigene Family Osteopontin Proteins/chemistry,immunology Sequence Alignment Sialoglycoproteins/chemistry,immunology,urine Urinary Bladder Calculi/chemistry
Chemicals
Antibodies, Monoclonal Proteins SPP1 protein, human Sialoglycoproteins Osteopontin Calcium Oxalate Aspartic Acid
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Shiraga H
Department of Pediatrics, University of Pennsylvania, Philadelphia.
Min W
VanDusen W J
Clayman M D
Miner D
Terrell C H
Sherbotie J R
Foreman J W
Przysiecki C
Neilson E G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.1.426
Published
1992-01-01
Pages
426-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48250
Subset
IM
Grants
NIDDK NIH HHS · DK-07006 · United States
NIDDK NIH HHS · DK-30280 · United States
NIDDK NIH HHS · DK-33501 · United States
Databases
GENBANK
M83248
External Links
PubMed source DOI: 10.1073/pnas.89.1.426 PMC Full Text View journal details
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