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PMID: 1730765 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Leishmania promastigotes require opsonic complement to bind to the human leukocyte integrin Mac-1 (CD11b/CD18).

The Journal of cell biology ·Vol. 116 ·No. 2 ·1992-01-00 ·Pages 511-20

Mosser DM, Springer TA, Diamond MS

Abstract

Previous reports have suggested that Leishmania spp. interact with macrophages by binding to Mac-1 (CD1 1b/CD18), a member of the leukocyte integrin family. To better define this interaction, we tested the ability of leishmania promastigotes to bind to purified leukocyte integrins and to cloned integrins expressed in COS cells. We show that leishmania promastigotes bind to cellular or purified Mac-1 but not lymphocyte function-associated antigen-1 in a specific, dose-dependent manner that requires the presence of serum. Binding is inhibited with specific monoclonal antibodies to Mac-1. In the absence of complement opsonization, three different species of leishmania tested fail to bind directly to any of the three leukocyte integrins. We show that binding to Mac-1 requires the third component of complement (C3). Organisms incubated in heat-inactivated serum or serum that has been immunologically depleted of C3 fail to bind to Mac-1. Because the addition of purified C3 to C3-depleted serum restores leishmania binding to Mac-1, we suggest that parasites gain entry into macrophages by fixing complement and subverting a well-characterized adhesive interaction in the immune system between Mac-1 and iC3b.

MeSH Terms
Animals Antibodies, Monoclonal Cell Adhesion Cells, Cultured Chlorocebus aethiops Complement C3/metabolism Complement System Proteins/metabolism Glycosphingolipids/metabolism Humans In Vitro Techniques Integrins/metabolism Leishmania/immunology Lymphocyte Function-Associated Antigen-1/metabolism Macrophage-1 Antigen/metabolism Macrophages/metabolism Opsonin Proteins/metabolism Recombinant Proteins Transfection
Chemicals
Antibodies, Monoclonal Complement C3 Glycosphingolipids Integrins Lymphocyte Function-Associated Antigen-1 Macrophage-1 Antigen Opsonin Proteins Recombinant Proteins lipophosphonoglycan Complement System Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mosser D M
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Springer T A
Diamond M S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.116.2.511
Published
1992-01-00
Pages
511-20
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289275
Subset
IM
Grants
NIGMS NIH HHS · T32 GM007753 · United States
NIAID NIH HHS · AI24313 · United States
NCI NIH HHS · CA31799 · United States
NIGMS NIH HHS · T32GM07753-11 · United States
External Links
PubMed source DOI: 10.1083/jcb.116.2.511 PMC Full Text View journal details
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