Abstract
The role of the mouse tumor necrosis factor (TNF) promoter, 5' untranslated region (UTR), and 3' UTR in TNF gene expression has been examined in three nonmacrophage cell lines (HeLa, NIH 3T3, and L-929). The TNF promoter is not macrophage-specific. On the contrary, it constitutively drives reporter gene expression in all three cell lines. Not only the full-length promoter but also truncated versions of the promoter, lacking NF-kappa B binding motifs, are active in each type of cell. The TNF 3' UTR effectively cancels reporter gene expression in HeLa cells and in NIH 3T3 cells but fails to block expression in L-929 cells. L-929 cells contain a factor that overcomes the inhibitory influence of the TNF 3' UTR. Its action depends upon the presence of sequences found in the TNF 5' UTR. Cell-fusion experiments reveal that this activator is trans-dominant. These studies highlight the essential role played by the TNF 3' UTR, which silences the TNF gene in cells that might otherwise express TNF. They also reveal the existence of an escape mechanism whereby inappropriate synthesis of TNF might occur.
MeSH Terms
3T3 Cells
Animals
Cell Fusion
Gene Expression Regulation
HeLa Cells
Humans
L Cells
Mice
Promoter Regions, Genetic
RNA, Messenger/genetics
Trans-Activators
Transcription, Genetic
Tumor Necrosis Factor-alpha/genetics
Chemicals
RNA, Messenger
Trans-Activators
Tumor Necrosis Factor-alpha
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kruys V
Howard Hughes Medical Institute, Dallas, TX 75235-9050.
Kemmer K
Shakhov A
Jongeneel V
Beutler B
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