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PMID: 17314305 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Estrogen mobilizes a subset of estrogen receptor-alpha-immunoreactive vesicles in inhibitory presynaptic boutons in hippocampal CA1.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 27 ·No. 8 ·2007-02-21 ·Pages 2102-11

Hart SA, Snyder MA, Smejkalova T, Woolley CS

Abstract

Although the classical mechanism of estrogen action involves activation of nuclear transcription factor receptors, estrogen also has acute effects on neuronal signaling that occur too rapidly to involve gene expression. These rapid effects are likely to be mediated by extranuclear estrogen receptors associated with the plasma membrane and/or cytoplasmic organelles. Here we used a combination of serial-section electron microscopic immunocytochemistry, immunofluorescence, and Western blotting to show that estrogen receptor-alpha is associated with clusters of vesicles in perisomatic inhibitory boutons in hippocampal CA1 and that estrogen treatment mobilizes these vesicle clusters toward synapses. Estrogen receptor-alpha is present in approximately one-third of perisomatic inhibitory boutons, and specifically in those that express cholecystokinin, not parvalbumin. We also found a high degree of extranuclear estrogen receptor-alpha colocalization with neuropeptide Y. Our results suggest a novel mode of estrogen action in which a subset of vesicles within a specific population of inhibitory boutons responds directly to estrogen by moving toward synapses. The mobilization of these vesicles may influence acute effects of estrogen mediated by estrogen receptor-alpha signaling at inhibitory synapses.

MeSH Terms
Animals Blotting, Western Cell Nucleus/metabolism Cholecystokinin/metabolism Estrogen Receptor alpha/metabolism Estrogens/pharmacology Female Fluorescent Antibody Technique Glutamate Decarboxylase/metabolism Hippocampus/metabolism,physiology,ultrastructure Immunohistochemistry In Vitro Techniques Microscopy, Electron Neural Inhibition/physiology Neurons/metabolism Neuropeptide Y/metabolism Parvalbumins/metabolism Presynaptic Terminals/metabolism,physiology,ultrastructure Rats Rats, Sprague-Dawley Synaptic Vesicles/drug effects,physiology Tissue Distribution
Chemicals
Estrogen Receptor alpha Estrogens Neuropeptide Y Parvalbumins Cholecystokinin Glutamate Decarboxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hart Sharron A
Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208, USA.
Snyder Melissa A
Smejkalova Tereza
Woolley Catherine S
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.5436-06.2007
Published
2007-02-21
Pages
2102-11
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6673535
Subset
IM
Grants
NINDS NIH HHS · NS37324 · United States
NCRR NIH HHS · RR015497 · United States
External Links
PubMed source DOI: 10.1523/JNEUROSCI.5436-06.2007 PMC Full Text View journal details
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