Home LiteratureArticle Details
PMID: 1732748 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein tyrosine phosphatase containing SH2 domains: characterization, preferential expression in hematopoietic cells, and localization to human chromosome 12p12-p13.

Molecular and cellular biology ·Vol. 12 ·No. 2 ·1992-02-00 ·Pages 836-46

Yi TL, Cleveland JL, Ihle JN

Abstract

Protein tyrosine phosphorylation has been implicated in the growth and functional responses of hematopoietic cells. Recently, approaches have been developed to characterize the protein tyrosine phosphatases that may contribute to regulation of protein tyrosine phosphorylation. One novel protein tyrosine phosphatase was expressed predominantly in hematopoietic cells. Hematopoietic cell phosphatase encodes a 68-kDa protein that contains a single phosphatase conserved domain. Unlike other known protein tyrosine phosphatases, hematopoietic cell phosphatase contains two src homology 2 domains. We also cloned the human homolog, which has 95% amino acid sequence identity. Both the murine and human gene products have tyrosine-specific phosphatase activity, and both are expressed predominantly in hematopoietic cells. Importantly, the human gene maps to chromosome 12 region p12-p13. This region is associated with rearrangements in approximately 10% of cases of acute lymphocytic leukemia in children.

Related Genes
HCP c-src
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Cell Line Chromosomes, Human, Pair 12 Cloning, Molecular Gene Expression Genes, src/genetics Hematopoietic Stem Cells Humans Kinetics Molecular Sequence Data Phosphorylation Protein Tyrosine Phosphatases/genetics,metabolism Sequence Alignment Sequence Homology, Nucleic Acid
Chemicals
Protein Tyrosine Phosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yi T L
Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
Cleveland J L
Ihle J N
References (87)
87 references, click to expand
  1. High-resolution chromosomal localization of human genes for amylase, proopiomelanocortin, somatostatin, and a DNA fragment (D3S1) by in situ hybridization.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6932-6 PMID: 6196780
  2. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  3. Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3623-7 PMID: 6304726
  4. Phosphorylation sites in enolase and lactate dehydrogenase utilized by tyrosine protein kinases in vivo and in vitro.
    J Biol Chem. 1984 Jun 25;259(12):7835-41 PMID: 6330085
  5. Nucleotide sequence of Fujinami sarcoma virus: evolutionary relationship of its transforming gene with transforming genes of other sarcoma viruses.
    Cell. 1982 Oct;30(3):787-95 PMID: 6291784
  6. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  7. Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase.
    Cell. 1991 Apr 5;65(1):91-104 PMID: 1707345
  8. T-lymphocyte interleukin 2-dependent tyrosine protein kinase signal transduction involves the activation of p56lck.
    Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1996-2000 PMID: 2000405
  9. Protein tyrosine phosphatases: a diverse family of intracellular and transmembrane enzymes.
    Science. 1991 Jul 26;253(5018):401-6 PMID: 1650499
  10. Structural diversity and evolution of human receptor-like protein tyrosine phosphatases.
    EMBO J. 1990 Oct;9(10):3241-52 PMID: 2170109
  11. Molecular characterization of a protein-tyrosine-phosphatase enriched in striatum.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7242-6 PMID: 1714595
  12. Association of B cell antigen receptor with protein tyrosine kinase Lyn.
    Science. 1991 Jan 11;251(4990):192-4 PMID: 1702903
  13. Tyrosine phosphatase CD45 is required for T-cell antigen receptor and CD2-mediated activation of a protein tyrosine kinase and interleukin 2 production.
    Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2037-41 PMID: 1672451
  14. Induction of tyrosine phosphorylation by the erythropoietin receptor correlates with mitogenesis.
    Mol Cell Biol. 1991 Oct;11(10):4895-902 PMID: 1656216
  15. Identification, cloning, and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to cytoskeletal protein 4.1.
    Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5867-71 PMID: 1648233
  16. Isolation of a cDNA clone encoding a human protein-tyrosine phosphatase with homology to the cytoskeletal-associated proteins band 4.1, ezrin, and talin.
    Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):5949-53 PMID: 1648725
  17. A Tyr/Ser protein phosphatase encoded by vaccinia virus.
    Nature. 1991 Mar 28;350(6316):359-62 PMID: 1848923
  18. T cell antigen receptor activation pathways: the tyrosine kinase connection.
    Cell. 1991 Mar 8;64(5):875-8 PMID: 1848158
  19. Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification.
    Blood. 1991 Nov 1;78(9):2222-8 PMID: 1932742
  20. New recurring chromosomal translocations in childhood acute lymphoblastic leukemia.
    Blood. 1991 May 1;77(9):2016-22 PMID: 2018838
  21. Hematopoietic cells express two forms of lyn kinase differing by 21 amino acids in the amino terminus.
    Mol Cell Biol. 1991 May;11(5):2391-8 PMID: 2017160
  22. Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence.
    Cell. 1991 Feb 8;64(3):615-23 PMID: 1991323
  23. Proliferative action of erythropoietin is associated with rapid protein tyrosine phosphorylation in responsive B6SUt.EP cells.
    J Biol Chem. 1991 Jan 5;266(1):609-14 PMID: 1985918
  24. Novel putative protein tyrosine phosphatases identified by the polymerase chain reaction.
    FEBS Lett. 1990 Oct 1;271(1-2):178-80 PMID: 2171999
  25. The colony stimulating factor-1 receptor associates with and activates phosphatidylinositol-3 kinase.
    Nature. 1989 Dec 7;342(6250):699-702 PMID: 2556641
  26. Molecular cloning of two types of GAP complementary DNA from human placenta.
    Science. 1988 Dec 23;242(4886):1697-700 PMID: 3201259
  27. Cloning of bovine GAP and its interaction with oncogenic ras p21.
    Nature. 1988 Sep 1;335(6185):90-3 PMID: 2842690
  28. The lck tyrosine protein kinase interacts with the cytoplasmic tail of the CD4 glycoprotein through its unique amino-terminal domain.
    Cell. 1989 Nov 17;59(4):627-36 PMID: 2582490
  29. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  30. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
    J Mol Biol. 1986 May 5;189(1):113-30 PMID: 3537305
  31. A family of receptor-linked protein tyrosine phosphatases in humans and Drosophila.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8698-702 PMID: 2554325
  32. Interleukin 3-specific tyrosine phosphorylation of a membrane glycoprotein of Mr 150,000 in multi-factor-dependent myeloid cell lines.
    EMBO J. 1987 Dec 20;6(13):3979-84 PMID: 3502088
  33. tdic(9;12): a nonrandom chromosome abnormality in childhood B-cell precursor acute lymphoblastic leukemia: a Pediatric Oncology Group Study.
    Blood. 1987 Dec;70(6):1962-5 PMID: 3499945
  34. Abnormalities of chromosome 12p13 and malignant proliferation of eosinophils: a nonrandom association.
    Br J Haematol. 1987 Sep;67(1):25-31 PMID: 3478077
  35. Nonrandom involvement of the 12p12 breakpoint in chromosome abnormalities of childhood acute lymphoblastic leukemia.
    Blood. 1986 Jul;68(1):69-75 PMID: 3459561
  36. Interaction of the unique N-terminal region of tyrosine kinase p56lck with cytoplasmic domains of CD4 and CD8 is mediated by cysteine motifs.
    Cell. 1990 Mar 9;60(5):755-65 PMID: 2107025
  37. Short related sequences in the cytoplasmic domains of CD4 and CD8 mediate binding to the amino-terminal domain of the p56lck tyrosine protein kinase.
    Mol Cell Biol. 1990 May;10(5):1853-62 PMID: 2109184
  38. Cloning of three human tyrosine phosphatases reveals a multigene family of receptor-linked protein-tyrosine-phosphatases expressed in brain.
    Proc Natl Acad Sci U S A. 1990 Sep;87(18):7000-4 PMID: 2169617
  39. Distinct functional roles of the two intracellular phosphatase like domains of the receptor-linked protein tyrosine phosphatases LCA and LAR.
    EMBO J. 1990 Aug;9(8):2399-407 PMID: 1695146
  40. Binding of transforming protein, P47gag-crk, to a broad range of phosphotyrosine-containing proteins.
    Science. 1990 Jun 22;248(4962):1537-9 PMID: 1694307
  41. Multiple hematopoietic growth factors signal through tyrosine phosphorylation.
    Growth Factors. 1990;2(2-3):213-20 PMID: 1692720
  42. Expression of a human T-cell protein-tyrosine-phosphatase in baby hamster kidney cells.
    Proc Natl Acad Sci U S A. 1990 Sep;87(18):7280-4 PMID: 2169624
  43. src-related tyrosine protein kinases as signaling components in hematopoietic cells.
    Cancer Cells. 1990 Oct;2(10):303-10 PMID: 1704247
  44. SH2 and SH3 domains: elements that control interactions of cytoplasmic signaling proteins.
    Science. 1991 May 3;252(5006):668-74 PMID: 1708916
  45. A protein-tyrosine phosphatase with sequence similarity to the SH2 domain of the protein-tyrosine kinases.
    Nature. 1991 Aug 22;352(6337):736-9 PMID: 1652101
  46. Cloning of PI3 kinase-associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases.
    Cell. 1991 Apr 5;65(1):83-90 PMID: 1849461
  47. cDNA cloning of a novel 85 kd protein that has SH2 domains and regulates binding of PI3-kinase to the PDGF beta-receptor.
    Cell. 1991 Apr 5;65(1):75-82 PMID: 1849460
  48. Interactions of phosphatidylinositol kinase, GTPase-activating protein (GAP), and GAP-associated proteins with the colony-stimulating factor 1 receptor.
    Mol Cell Biol. 1990 Nov;10(11):5601-8 PMID: 2172781
  49. Protein tyrosine phosphatase domains from the protochordate Styela plicata.
    Immunogenetics. 1991;33(1):33-41 PMID: 1704870
  50. Interaction of the IL-2 receptor with the src-family kinase p56lck: identification of novel intermolecular association.
    Science. 1991 Jun 14;252(5012):1523-8 PMID: 2047859
  51. Role of myc in the abrogation of IL3 dependence of myeloid FDC-P1 cells.
    Oncogene Res. 1987 Aug;1(3):279-96 PMID: 2453016
  52. A new member of the immunoglobulin superfamily that has a cytoplasmic region homologous to the leukocyte common antigen.
    J Exp Med. 1988 Nov 1;168(5):1523-30 PMID: 2972792
  53. Inositol phospholipid-specific phospholipase C: complete cDNA and protein sequences and sequence homology to tyrosine kinase-related oncogene products.
    Proc Natl Acad Sci U S A. 1988 Aug;85(15):5419-23 PMID: 2840660
  54. Human placenta protein-tyrosine-phosphatase: amino acid sequence and relationship to a family of receptor-like proteins.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5252-6 PMID: 2546149
  55. Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase.
    Biochemistry. 1988 Nov 29;27(24):8695-701 PMID: 2853967
  56. Sequence similarity of phospholipase C with the non-catalytic region of src.
    Nature. 1988 Mar 17;332(6161):269-72 PMID: 2831461
  57. Purification of the major protein-tyrosine-phosphatases of human placenta.
    J Biol Chem. 1988 May 15;263(14):6722-30 PMID: 2834386
  58. The leukocyte common antigen (CD45): a putative receptor-linked protein tyrosine phosphatase.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7182-6 PMID: 2845400
  59. A noncatalytic domain conserved among cytoplasmic protein-tyrosine kinases modifies the kinase function and transforming activity of Fujinami sarcoma virus P130gag-fps.
    Mol Cell Biol. 1986 Dec;6(12):4396-408 PMID: 3025655
  60. Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):9138-42 PMID: 2973607
  61. Neoplastic transformation of mast cells by Abelson-MuLV: abrogation of IL-3 dependence by a nonautocrine mechanism.
    Cell. 1985 Jul;41(3):685-93 PMID: 2988783
  62. Malignant transformation of a growth factor-dependent myeloid cell line by Abelson virus without evidence of an autocrine mechanism.
    Cell. 1985 Jul;41(3):677-83 PMID: 2988782
  63. Structural variants of human T200 glycoprotein (leukocyte-common antigen).
    EMBO J. 1987 May;6(5):1251-7 PMID: 2956090
  64. Stimulation of factor-dependent myeloid cell lines with interleukin 3 induces tyrosine phosphorylation of several cellular substrates.
    J Biol Chem. 1988 Dec 15;263(35):19203-9 PMID: 2461935
  65. A novel viral oncogene with structural similarity to phospholipase C.
    Nature. 1988 Mar 17;332(6161):272-5 PMID: 2450282
  66. Vanadate can replace interleukin 3 for transient growth of factor-dependent cells.
    Exp Cell Res. 1987 Jul;171(1):16-23 PMID: 2442014
  67. Tyrosine kinase oncogenes abrogate interleukin-3 dependence of murine myeloid cells through signaling pathways involving c-myc: conditional regulation of c-myc transcription by temperature-sensitive v-abl.
    Mol Cell Biol. 1989 Dec;9(12):5685-95 PMID: 2555703
  68. cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family.
    Proc Natl Acad Sci U S A. 1989 Jul;86(14):5257-61 PMID: 2546150
  69. Evidence that the leukocyte-common antigen is required for antigen-induced T lymphocyte proliferation.
    Cell. 1989 Sep 22;58(6):1055-65 PMID: 2550143
  70. The leukocyte common antigen family.
    Annu Rev Immunol. 1989;7:339-69 PMID: 2523715
  71. Cloning of the murine c-fgr proto-oncogene cDNA and induction of c-fgr expression by proliferation and activation factors in normal bone marrow-derived monocytic cells.
    Oncogene. 1989 Sep;4(9):1081-7 PMID: 2674853
  72. Selection of lineage-restricted cell lines immortalized at different stages of hematopoietic differentiation from the murine cell line 32D.
    J Cell Biol. 1989 Aug;109(2):833-41 PMID: 2668305
  73. Signal transduction through the CD4 receptor involves the activation of the internal membrane tyrosine-protein kinase p56lck.
    Nature. 1989 Mar 16;338(6212):257-9 PMID: 2784195
  74. Selective growth response to IL-3 of a human leukaemic cell line with megakaryoblastic features.
    Br J Haematol. 1988 Jul;69(3):359-66 PMID: 3261598
  75. The CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56lck.
    Cell. 1988 Oct 21;55(2):301-8 PMID: 3262426
  76. Hematopoietic growth factors activate the tyrosine phosphorylation of distinct sets of proteins in interleukin-3-dependent murine cell lines.
    Mol Cell Biol. 1988 May;8(5):2214-8 PMID: 3260330
  77. Reversible dependence on growth factor interleukin-3 in myeloid cells expressing temperature sensitive v-abl oncogene.
    Oncogene Res. 1988 Feb;2(3):277-84 PMID: 3259302
  78. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
  79. Signal transduction of the human granulocyte-macrophage colony-stimulating factor and interleukin-3 receptors involves tyrosine phosphorylation of a common set of cytoplasmic proteins.
    Blood. 1990 Aug 15;76(4):706-15 PMID: 2166606
  80. Cloning and expression of a widely expressed receptor tyrosine phosphatase.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6112-6 PMID: 2166945
  81. Molecular cloning and chromosome mapping of the human gene encoding protein phosphotyrosyl phosphatase 1B.
    Proc Natl Acad Sci U S A. 1990 Jul;87(13):5148-52 PMID: 2164224
  82. CD45, an integral membrane protein tyrosine phosphatase. Characterization of enzyme activity.
    J Biol Chem. 1990 Jun 25;265(18):10674-80 PMID: 2162357
  83. Cloning of a cDNA for a major human protein-tyrosine-phosphatase.
    Proc Natl Acad Sci U S A. 1990 Apr;87(7):2735-9 PMID: 2157211
  84. Cloning and expression of a protein-tyrosine-phosphatase.
    Proc Natl Acad Sci U S A. 1990 Feb;87(4):1501-5 PMID: 2154749
  85. Association of the fyn protein-tyrosine kinase with the T-cell antigen receptor.
    Proc Natl Acad Sci U S A. 1990 Jun;87(11):4358-62 PMID: 2190221
  86. Interleukin 2-induced tyrosine phosphorylation. Interleukin 2 receptor beta is tyrosine phosphorylated.
    J Biol Chem. 1990 Feb 25;265(6):3561-7 PMID: 2303462
  87. Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus.
    Cell. 1983 Mar;32(3):881-90 PMID: 6299580
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.2.836-846.1992
Published
1992-02-00
Pages
836-46
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364317
Subset
IM
Grants
NCI NIH HHS · P30 CA21765 · United States
NIDDK NIH HHS · R01 DK42932 · United States
Databases
GENBANK
M68902, M74903
External Links
PubMed source DOI: 10.1128/mcb.12.2.836-846.1992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2